| 摘要: |
| 本文在实验室条件下通过静态水质染毒方式在生理生化和转录水平上探究了我国重要经济鱼种鲤鱼（Cyprinus carpioio）对多氯联苯Aroclor 1242的氧化应激：鲤鱼肝脏组织抗氧化酶[超氧化物歧化酶（Superoxide Dismutase，SOD）和过氧化氢酶（Catalase，CAT）]活性、脂质过氧化产物丙二醛（Malonydialdehyde，MDA）含量、相关抗氧化酶编码基因（Cu/Zn-SOD、Mn-SOD、CAT）、应激蛋白HSP70、转录因子Nrf2和芳烃受体AhR2基因表达。结果表明：Aroclor 1242胁迫下，鲤鱼肝脏组织抗氧化酶活性表现出低浓度促进高浓度抑制的趋势；肝脏组织丙二醛含量显著升高，其中390μg/L暴露组鲤鱼肝脏组织MDA含量在第6d高于对照组92.1%（P<0.01），表明肝脏组织发生脂质过氧化，膜系统受到损伤；鲤鱼肝脏组织中Cu/Zn-SOD、Mn-SOD、CAT、HSP70、Nrf2和AhR2编码基因表达出现不同程度下调。本文从酶学和基因转录水平上揭示了Aroclor 1242胁迫下鲤鱼肝脏组织细胞的氧化应激。 |
| 关键词: 多氯联苯 鲤鱼 氧化应激 脂质过氧化 基因表达 |
| DOI：10.11693/hyhz20180700170 |
| 分类号:Q958.8 |
| 基金项目:国家自然科学基金，31200400号，31500414号；山东省自然科学基金，ZR2017MC037号。 |
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| OXIDATIVE STRESS IN LIVER OF CYPRINUS CARPIOIO EXPOSED TO PCBS AROCLOR 1242 |
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WANG Ren-Jun, WANG Jian-Guo, DING Ning, SUN Meng, GAO Pei-Ke
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College of Life Sciences, Qufu Normal University, Qufu 273165, China
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| Abstract: |
| We investigated the effects of polychlorinated biphenyls Aroclor 1242 on the oxidative stress of Cyprinus carpioio liver under static water exposure in the laboratory. The activities of antioxidant enzymes (SOD, CAT), content of lipid peroxidation products (MDA), expression of genes encoding Cu/Zn-SOD, Mn-SOD, and CAT, heat shock protein 70 (HSP70), nuclear factor erythroid 2-related factor 2 (Nrf2), and Aryl hydrocarbon receptor 2 (AhR2) were analyzed. The results show that Aroclor 1242 changed significantly the SOD and CAT activities in the liver, showing promotion at low concentration and inhibition at high concentration. Aroclor 1242 significantly increased MDA content in the liver tissue. Compared to the control, the MDA content was 77.5% higher in the liver exposed to 390μg/L for three days, indicating that Aroclor 1242 induced strong lipid peroxidation. After exposure to medium-high concentrations of Aroclor 1242 (78, 156, and 390μg/L) for 8 days, the expression of antioxidant enzyme encoding gene Cu/Zn-SOD, Mn-SOD, CAT, heat shock protein 70 (HSP70), and Nrf2 and AhR2 genes were down-regulated in the liver tissue of the carp. Collectively, this study revealed the influences of Aroclor 1242 on oxidative stress of Cyprinus carpioio liver from the perspectives of enzyme and gene transcription levels. |
| Key words: Polychlorinated biphenyls C.carpio oxidative stress lipid peroxidation gene expression |
