| 摘要: |
| 在形态分类的基础上，采用多重PCR （Multiplex Species-Specific PCR）和DNA序列分析，基于细胞色素氧化酶Ⅰ（cytochrome oxidase I，COI）基因构建系统进化树鉴定牡蛎种类及确定其分类地位，研究了广东雷州半岛东部沿岸牡蛎科（Ostreidae）常见牡蛎的种类及分布。本次研究从雷州半岛东部沿岸的11个地点采集了363个野生牡蛎样品，并对全部样品进行了形态分析和多重PCR的鉴定，对未鉴定出来的114个样品进行COI基因的扩增并测序，构建系统进化树进行种类分析，共检测出3属5种牡蛎。巨蛎属（Crassostrea）有两种，分别为熊本牡蛎（C.sikamea）185个和福建牡蛎（C.gigas angulata）109个；小蛎属（Saccostrea）有两种，棘刺牡蛎（S.echinata）48个和团聚牡蛎（S. malabonensis）19个，牡蛎属未定种Ostrea sp.1有2个。结果表明，雷州半岛东部沿岸牡蛎种类丰富，至少有5种牡蛎分布于潮间带，其中优势种为熊本牡蛎和福建牡蛎，分布范围比较广。小蛎属和牡蛎属牡蛎数量较少，分布比较集中。 |
| 关键词: 雷州半岛 牡蛎科 多重PCR COI |
| DOI：10.11693/hyhz20180300054 |
| 分类号:Q959.215 |
| 基金项目:国家自然科学基金项目，41776179号，40406032号，40876084号；现代农业产业技术体系建设专项资金，CARS-47号；中国科学院战略性先导科技专项，XDA10020305号；国家科技基础性工作专项，2014FY110500号；大学生创新创业训练计划项目，201710065032号。 |
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| CLASSIFICATION AND DISTRIBUTION OF OYSTERS IN EASTERN COAST OF LEIZHOU PENINSULA, CHINA |
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CUI Zong-Mei1,2, HUANG Jin-Wei3, WANG Hai-Yan1, HU Li-Sha1,2, LIU Yu-Meng1,2
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1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Tianjin Normal University, Tianjin 300387, China
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| Abstract: |
| We used multiplex species-specific PCR and DNA barcoding to identified oysters in the eastern coast of Leizhou Peninsula, Guangdong, China. A total of 363 oysters were collected from 11 sites. All the oysters were identified by multiplex species-specific PCR and 114 oysters were identified in mitochondrial COI gene-based analysis. At least five oyster species were recognized. Two Crassostrea species were dominated and widely distributed:C. sikamea (185 samples) and C. gigas angulata (109 samples); two Saccostrea species:S. echinata (48 samples) and S. malabonensis (19 samples), limited in certain places, and 2 samples belonged to genus Ostrea sp.1. |
| Key words: Leizhou Peninsula Ostreidae multiplex species-specific PCR COI |
