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| 不同氮浓度条件下红色赤潮藻脂肪酸含量及组成特征 |
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刘云1,2,3, 陈田田1,2,3, 宋书群1,2,3, 李才文1,2,3,4
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1.中国科学院海洋研究所 海洋生态与环境科学重点实验室 青岛 266071;2.青岛海洋科学与技术试点国家实验室 海洋生态与环境科学功能实验室 青岛 266237;3.中国科学院海洋大科学研究中心 青岛 266071;4.中国科学院大学 北京 100049
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| 摘要: |
| 在不同氮浓度条件下对红色赤潮藻（Akashiwo sanguinea）进行了一次性培养，研究了其生长过程中脂肪酸含量及组成变化。结果表明：红色赤潮藻脂肪酸含量及组成受初始氮浓度及生长阶段的影响。低氮处理组（0—25μmol/L）脂肪酸含量[（150.02±54.84）—（166.83±46.46）mg/g dw]显著高于其他处理组（P<0.05），883μmol/L组最低，约为（86.70±5.66）mg/g dw；各培养组脂肪酸含量随着培养时间的延长而增加，稳定期时脂肪酸含量最高，指数生长前期含量最低。红色赤潮藻含28种脂肪酸，以C16：0、C18：1、C20：5ω3（EPA）和C22：6ω3（DHA）为主，且DHA相对含量较高，最高可占总脂肪酸含量的30.46%。随着初始氮浓度的增加，红色赤潮藻饱和脂肪酸（SFA）和单不饱和脂肪酸（MUFA）在总脂肪酸中所占比例下降，而多不饱和脂肪酸（PUFA）所占比例升高。在红色赤潮藻生长过程中，MUFA相对含量在稳定期时较高，而PUFA相对含量在指数生长前期较高。研究结果可为进一步揭示红色赤潮藻赤潮应对环境胁迫的生理响应机制提供依据。 |
| 关键词: 脂肪酸 红色赤潮藻 氮浓度 生长阶段 |
| DOI：10.11693/hyhz20181200284 |
| 分类号:X55 |
| 基金项目:国家自然科学基金项目，41506141号；青岛海洋科学与技术试点国家实验室海洋生态与环境科学功能实验室青年人才培育项目，LMEES-YTSP-2018-01-06号；国家基金委—山东省联合基金项目，U1606404号。 |
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| CONTENT AND COMPOSITION OF FATTY ACID IN PLANKTONIC DINOFLAGELLATE AKASHIWO SANGUINEA CULTURED IN DIFFERENT NITRATE CONCENTRATIONS |
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LIU Yun1,2,3, CHEN Tian-Tian1,2,3, SONG Shu-Qun1,2,3, LI Cai-Wen1,2,3,4
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1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Science, Qingdao 266071, China;2.Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;4.University of Chinese Academy of Science, Beijing 100049, China
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| Abstract: |
| Batch cultures of the planktonic dinoflagellate Akashiwo sanguinea were conducted in different nitrate concentrations. Variations of the fatty acid content and its composition at different growth stages of A. sanguinea were analyzed. The results indicate that the fatty acid content and composition varied with both N concentration and growth stage. In particular, the fatty acid contents in lower nitrogen concentrations[0-25μmol/L, (150.02±54.84)- (166.83±46.46)mg/g dw] were significantly higher than those in other N treatments (P<0.05). The lowest value (86.70±5.66)mg/g dw was found in the 883μmol/L treatment. The fatty acid content in each treatment increased with the growth of A. sanguinea, reaching the highest in the stationary growth phase and lowest in the early exponential growth phase. Twenty-eight 28 fatty acids were detected in A. sanguinea, including mainly C16:0, C18:1, C20:5ω3 (EPA), and C22:6ω3 (DHA). The DHA content was relatively higher, with the maximum of 30.46% of total fatty acids. With the increases of initial N addition, the proportions of saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) declined whereas the polyunsaturated fatty acid (PUFA) increased. In addition, the proportion of MUFA was higher in the stationary phase, while PUFA was higher in the early exponential growth phase. The results help further understanding of the physiological strategy of A. sanguinea adoption to an environmental stress. |
| Key words: fatty acid Akashiwo sanguinea nitrogen concentration growth stage |