| 摘要: |
| 虫黄藻是珊瑚礁生态系统中最主要的初级生产者，并在珊瑚礁的建造过程中起着关键作用。为探究虫黄藻对温度和光照的生理响应，本研究以E型虫黄藻（Symbiodinium voratum）为实验对象，通过室内培养，并结合谢尔福德耐受性定律建立了基于温度的虫黄藻生长速率的耐受性模型。结果显示：E型虫黄藻在光强90μE培养下，最适生长温度为22.56℃，适温范围为16.72—28.40℃；温度培养实验中，23℃时，虫黄藻中有机碳（C）、氮（N）积累最多； 27℃时，虫黄藻PSⅡ的原初光能转化效率（Fv/Fm）显著高于其余两个温度组；光照培养实验中，E型虫黄藻在温度23℃培养下，光强的适宜范围为100—200μE；并通过提高细胞内叶绿素a含量和Fv/Fm两种方式应对外界光照不足。 |
| 关键词: 虫黄藻 温度 光强 生理响应 |
| DOI：10.11693/hyhz20180900229 |
| 分类号:Q142.9 |
| 基金项目:国家科技基础资源调查专项，2017FY201404号；科技部基础性工作专项“我国近海重要海草资源及生境调查”项目，2015FY110600号；国家自然科学基金，41806198号；公益性行业（农业）科研专项，201403008号。 |
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| PHYSIOLOGICAL RESPONSES OF SYMBIODINIUM VORATUM TO TEMPERATURE AND LIGHT INTENSITY |
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ZHAO Zhen-Lu1,2, LIU Jia-Xing1, ZHANG Yue-Huan1, LIU Tian-Tian3, TAN Ye-Hui1,2
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1.Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China
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| Abstract: |
| The genus Symbiodinium is an important primary producer in coral reef ecosystem and plays a key role in coral reef construction. Yet knowledge of growth kinetics of these photosynthetic endosymbionts and their physiological responses is limited. To study the physiological responses to temperature and light intensity of Symbiodinium, Symbiodinium voratum (Clade E) was cultured in laboratory. We established a tolerance model between temperature and specific growth rate based on experimental results and the Shelford's Law of Tolerance. The results show that the temperature tolerance model agreed with the Shelford's Law of Tolerance. The optimum temperature and suitable temperature range of S. voratum growth were 22.56℃ and 16.72-28.40℃, respectively. The accumulations of organic carbon and nitrogen peaked at 23℃, and Fv/Fm (the maximum quantum efficiency of PSⅡ photochemistry) at 27℃. The suitable light intensity at 23℃ was 100-200μE, while at a lower light intensity, S. voratum could increase its chlorophyll a per cell and Fv/Fm in respond to the environmental change. |
| Key words: Symbiodinium voratum temperature light intensity physiological responses |
