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| 拟穴青蟹(Scylla paramamosain)六个地理群体肌肉氨基酸和脂肪酸的比较分析 |
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李永亮1,2, 赵欢欢1, 李荣华1,2, 王春琳1,2, 母昌考1,2, 宋微微1, 叶央芳1,2
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1.宁波大学 应用海洋生物学教育部重点实验室 宁波 315211;2.浙江海洋高效健康养殖协同创新中心 宁波 315211
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| 摘要: |
| 本文对广西钦州（Gq）、福建霞浦（Fx）、浙江温州（Zw）、浙江三门（Zs）、浙江宁海（Zn）、浙江慈溪（Zc）等6个野生群体拟穴青蟹（Scylla paramamosain）肌肉进行了氨基酸及脂肪酸分析。共测定出19种游离氨基酸和26种脂肪酸。氨基酸含量在80.94—191.39mg/g之间，Gq为最高；总脂肪酸含量在7.97—11.38mg/g之间，Zn为最高。主要氨基酸为甘氨酸和精氨酸；主要脂肪酸为C20：5n3（EPA），占总脂肪酸含量的平均值为（22.47±5.84）%。各群体风味氨基酸和多不饱和脂肪酸含量丰富，占总氨基酸比例（TDAA/TFAA）和总脂肪酸比例（PUFA/TFA）分别为（53.62±5.43）%和（59.27±5.29）%。Zs群体的必需氨基酸含量及C22：6n3（DHA）均为最高。主成分分析结果表明：各群体的氨基酸与脂肪酸组成与含量相似性与地理距离分布具有一定的相关性，特别是Gq群体，在氨基酸与脂肪酸的分类中与其他群体区分比较明显。各群体风味氨基酸和多不饱和脂肪酸的聚类分析结果均表明：Zw、Zc与Gq群体较为接近，具有较强的甜味； Fx、Zn与Zs群体较为接近，具有较强的香味及不饱和脂肪酸营养价值。 |
| 关键词: 拟穴青蟹 群体 游离氨基酸 脂肪酸 |
| DOI：10.11693/hyhz20181100282 |
| 分类号:Q523;S917 |
| 基金项目:浙江省农业重大科技专项，2016C02055-8号；现代农业产业技术体系专项，CARS-48号；宁波市科技富民项目，2016C10037号；宁波大学王宽诚幸福基金资助。 |
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| COMPARISON OF AMINO ACID AND FATTY ACID COMPOSITION IN SCYLLA PARAMAMOSAIN FROM SIX DIFFERENT WILD POPULATIONS |
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LI Yong-Liang1,2, ZHAO Huan-Huan1, LI Rong-Hua1,2, WANG Chun-Lin1,2, MU Chang-Kao1,2, SONG Wei-Wei1, YE Yang-Fang1,2
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1.Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China;2.Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China
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| Abstract: |
| In this study, we analyzed the amino acid composition and fatty acid composition of Scylla paramamosain collected from six different wild populations:Qinzhou, Guangxi Province (Gq); Xiapu, Fujian Province (Fx); Wenzhou, Zhejiang Province (Zw); Sanmen, Zhejiang Province (Zs); Ninghai, Zhejiang Province (Zn); Cixi, Zhejiang Province (Zc). A total of 19 free amino acids and 26 fatty acids were identified from 6 populations. The amino acid content ranged from 80.94-191.39mg/g, the highest amino acid content was detected in Gq population; the total fatty acid content ranged from 7.97-11.38mg/g, the highest fatty acid content was detected in Zn population. The main amino acids were glycine and arginine, and the main fatty acid was C20:5n3 (EPA), which accounted for (22.47±5.84)% of total fatty acids. There were abundant flavor amino acids and polyunsaturated fatty acids in all the populations, which accounted for (53.62±5.43)% of the total free amino acids (TDAA/TFAA) and (59.27±5.29)% of the total fatty acids (PUFA/TFA). The highest essential amino acid contents and C22:6n3 (DHA) were found in the Zs population. Principal component analysis showed that the composition and content of amino acids and fatty acids were correlated with geographical distribution of these populations; especially in Gq population, the similarities of amino acids and fatty acids were more distinct from other populations. The results of cluster analysis of flavor amino acids and polyunsaturated fatty acids showed that Zw, Zc and Gq populations were grouped in a clade and had strong sweetness, while Fx, Zn and Zs populations were grouped in another clade and had strong aroma and nutritional value of unsaturated fatty acids. |
| Key words: Scylla paramamosain populations free amino acids fatty acids |