| 摘要: |
| 本研究以确认鱼类早期个体规模化耳石锶（Sr）标记的可行性及其时滞特征为目的，选择鲤（Cyprinus carpio）仔鱼为实验对象，开展了六水氯化锶（SrCl2·6H2O）溶液2d的活体浸泡标记研究。结果显示，该浸泡标记过程对鲤存活、生长无显著影响。利用X射线电子探针微区分析（EPMA）技术进行分析：定量线分析结果显示对照组Sr/Ca比值（3.0左右）趋于稳定，标记组却均出现了明显的标记峰值（远>3.0）；面分布分析结果表明在标记组鲤耳石上均呈现出一圈明显、清晰可见的红色“高Sr标记环”。此外，对比对照组和标记组微化学结果发现，“高Sr标记环”在恢复续养250d后的鲤耳石仍然可见，说明Sr标记可长期存在。观察鲤仔鱼耳石微结构，发现Sr在耳石上存在明显的时滞效应。首次得出在开始浸泡后的第3天，耳石上才开始出现Sr标记，在浸泡结束后的第6天，耳石Sr标记区才会完全消失的结论。本研究首次揭示出，外源Sr标记的方法在鲤仔鱼种群大规模增殖放流标记和评价中将具有很强的稳定性、可靠性、可行性和应用潜力。 |
| 关键词: 鲤 耳石 锶标记 增殖放流 X射线电子探针微区分析 |
| DOI：10.11693/hyhz20190100003 |
| 分类号:S917.4 |
| 基金项目:中央级公益性科研院所基本科研业务费专项，2019JBFM06号；农业财政专项，CJDC-2017-22号。 |
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| CHARACTERCTERISTICS OF OTOLITH STRONTIUM MARKING AND ITS TIME LAGS OF LARVAL CYPRINUS CARPIO |
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QIU Chen1, JIANG Tao2, CHEN Xiu-Bao2, LIU Hong-Bo2, YANG Jian1,2
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1.Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;2.Key Laboratory of Fishery Ecological Environment Assessment and Research Conservation in Middle and Lower Reaches of the Yangtze River, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
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| Abstract: |
| We selected the larval common carp (Cyprinus carpio) as a targeted fish and immersed the fish in SrCl2·6H2O solution for 2d to understand the effect of Sr-marking and the time lag characteristics of the appearance and completion of corresponding marking. The results show no obvious effect of Sr-marking on mortality and growth of the fish. The marking rings on common carp otoliths were clear as detected by X-ray electron microprobe microanalysis (EPMA). The Sr/Ca ratio along line transects from the core (0μm) to the edge of the otoliths shows a significant elevated range in the treatment group. Meanwhile, a clear marking ring of high-Sr content is visible in otolith of Sr-marked C. carpio, and the ring remained detectable in 5-250 days after the immersion. In addition, time lags of 3- and 6-day were found between the first day of immersion and the day of Sr appearance in otolith, and between the last day of immersion and the day of Sr disappearance in otolith, respectively. The present approach of exogenous Sr-marking provides a useful otolith-marking tool for larval C. carpio, and it is stable, reliable, and feasible. |
| Key words: Cyprinus carpio otolith strontium marking artificial enhancement release X-ray electron probe microanalysis |
