| 摘要: |
| 精氨酸/赖氨酸特异性半胱氨酸蛋白酶（Metacaspase）在浮游植物程序性细胞死亡（programmed cell death，PCD）过程中起着关键作用。本文利用生物信息学方法分析metacaspase基因在浮游植物中的分布，并以海洋球石藻（Emiliania huxleyi）metacaspases（EhMCs）为代表分析其结构和功能，依据Uniprot和String数据库挖掘与metacaspase功能相关并参与浮游植物PCD过程的互作蛋白。结果发现：共计29条、四种类型的metacaspases分布于11种浮游植物中，它们的metacaspase蛋白一级结构存在明显差异，但位于p20的组氨酸和半胱氨酸活性位点均具有高度的保守性；EhMCs三级结构与酵母的metacaspase十分相似，暗示它们具有类似的功能；浮游植物metacaspases与多种蛋白存在直接或间接的互作关系，在细胞PCD过程、响应环境胁迫和能量代谢等方面发挥重要作用。另外，metacaspase的磷酸化修饰可能在浮游植物PCD信号转导过程中起调节作用。 |
| 关键词: 浮游植物 程序性细胞死亡(PCD) metacaspase 结构与功能 互作蛋白 |
| DOI：10.11693/hyhz20190300056 |
| 分类号:S432.1 |
| 基金项目:国家自然科学基金项目，41576166号；福建省自然科学基金项目，2019J01696号；福建省科技计划重点项目，2015Y0039号；福建省食品微生物与酶工程重点实验室开放基金项目，B18097-1号。 |
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| DISTRIBUTION, STRUCTURE, AND FUNCTION OF PHYTOPLANKTON METACASPASES |
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SU Jin-Jing1,2, CAI Wei-Cong1,2, LI Gui-Ling1, LI Jian1, ZENG Jun1, LIU Jing-Wen1,2
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1.College of Food and Bioengineering, Jimei University, Xiamen 361021, China;2.Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China
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| Abstract: |
| Metacaspases are evolutionarily distant homologs of caspases and play key roles in phytoplankton programmed cell death (PCD). We analyzed the characteristics of distribution, structure, and function of phytoplankton metacaspases using bioinformatics methods. The results show that a total of 29 phytoplankton metacaspases are found in 11 phytoplankton species, and can be grouped into three main types, namely typeⅠ, typeⅡ, and type Ⅲ based on their multiple alignment of amino acid sequence. Despite the low amino acid sequence identity, they displayed high structural conservation in histidine-and cysteine-containing catalytic domain. The three-dimensional structures of Emiliania huxleyi (EhMC) are very close to that of yeast metacaspase, suggesting that they might have similar function. The results of the protein-protein interaction analysis revealed that metacaspases directly or indirectly interacted with multiple cellular proteins, playing a critical role in cell apoptosis, response to environmental stress, and energy metabolism. Furthermore, the phosphorylation modification of metacaspase may play a regulatory role in the PCD signal transduction of phytoplankton. |
| Key words: phytoplankton programmed cell death (PCD) metacaspase structure and function protein-protein interaction |
