| 摘要: |
| 作为重要的水质参数，高浓度的氨氮对水生动物产生有害影响。氨氮的安全浓度对海水养殖物种至关重要。本文采用静水法，在盐度30.0±1.0，pH 7.8±0.2，温度20.8±0.5℃条件下，以荣成月湖海区的长蛸（Octopus minor）成体为研究对象，分析了水体氨氮对其急性毒性作用。通过预实验获得长蛸成体可耐受的浓度范围，从中选取浓度0（对照）、87.0、124.5、143.5、180.3、204.0、242.5mg/L等7个浓度梯度测定长蛸成体耐受性，采用概率单位法、寇氏法和线性回归法分别计算48h、72h和96h的半致死浓度（LC50）。实验结果显示，氨氮毒性效应随着浓度和胁迫时间的增长而增加，氨氮胁迫48h、72h和96h的半致死浓度分别为187.93、154.61和124.92mg/L（χ2检验P<0.05）；显示氨氮对长蛸成体具有显著毒性作用。与其他水生动物相比，长蛸对于氨氮的耐受性较强，对氨氮毒性的自我调节能力较强，具体的调节机制有待进一步研究。 |
| 关键词: 长蛸 氨氮 急性毒性 半致死浓度 |
| DOI：10.11693/hyhz20190700132 |
| 分类号: |
| 基金项目:国家自然科学基金项目，31672257号；中央高校基本科研业务费专项，201822022号。 |
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| DETERMINATION OF THE MEDIAN LETHAL CONCENTRATION AND ACUTE TOXICITY OF OCTOPUS MINOR UNDER DIFFERENT CONCENTRATIONS OF AMMONIA AND NITROGEN STRESS |
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CHEN Zhi-Wei1,2, XU Ran1,2, NAN Ze1,2, ZHENG Xiao-Dong1,2
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1.Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China;2.Key Laboratory of Mariculture, Ocean University of China, Qingdao 266003, China
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| Abstract: |
| As an important water quality parameter, ammonia nitrogen in high concentration has a detrimental effect on aquatic animals. The safe concentration of ammonia nitrogen is critical for marine aquaculture species. In this study, we used a static biological toxicity test to analyze the acute toxicity of ammonia nitrogen on Octopus minor adult in a lake in which the water salinity was 30.0±1.0, pH 7.8±0.2, and temperature 20.8±0.5℃. First, the toleration range of O. minor to ammonia nitrogen concentration was determined first in a pre-experiment. Second, the sub-lethal ammonia nitrogen exposure treatments were performed based on the toleration range at concentrations of 0 (control), 87.0, 124.5, 143.5, 180.3, 204.0, and 242.5mg/L for 48h, 72h, and 96h. At last, we applied the Probit analysis, Kouchi method, and linear regression to determine the sub-lethal concentration at each time point. The results show that the toxic effect of ammonia nitrogen increased with increases in the concentration and the time. The median lethal concentration of ammonia nitrogen at 48h, 72h, and 96h was 187.93, 154.61, and 124.92mg/L, respectively (P<0.05), showing greater toleration to ammonia nitrogen than that of other aquatic species, such as Apostichopus japonicus whose 96h median lethal concentration was 90.57mg/L. The safety concentration of ammonia nitrogen for O. minor was 12.49mg/L. In the future, the self-regulation of ammonia nitrogen toxicity and specific regulatory mechanism in O. minor should be explored. |
| Key words: Octopus minor ammonia nitrogen acute toxicity the median lethal concentration |
