| 本文已被：浏览 1555次 下载 799次 |
码上扫一扫！ |
|
|
| γ-氨基丁酸、氯化钾和5-羟色胺对羊鲍(Haliotis ovina)幼虫存活、附着和变态的影响 |
|
浦舒为1,2,3,4, 逯峰1,2,3,4, 王佳伟1,2,3,4, 黄勃1,2,3,4, 王小兵3,4, 王林桂3,4
|
|
1.海南大学海洋学院 海口 570228;2.海南省热带水生生物技术重点实验室 海口 570228;3.海南大学南海海洋资源利用国家重点实验室 海口 570228;4.海南大学热带生物资源教育部重点实验室 海口 570228
|
|
| 摘要: |
| 使用γ-氨基丁酸（γ-aminobutyric acid，GABA）、氯化钾和5-羟色胺，进行羊鲍（Haliotis ovina）幼虫存活、附着和变态过程中的诱导实验。结果表明，γ-氨基丁酸对羊鲍幼虫存活、附着及变态都有促进作用。γ-氨基丁酸浓度为10-4mol/L时处理72h的附着率（26.27%）和变态率（22.26%）高于其他组别，10-6mol/L处理72h存活率（30.84%）高于其他组别。氯化钾能在高浓度（10-2-10-3mol/L）下诱导附着，而对变态和存活没有影响。氯化钾浓度为10-3mol/L处理时间为24h和72h的附着率高于其他组别，分别为11.3%和16.4%。5-羟色胺可诱导附着和变态，对幼虫的存活无影响或有抑制作用。5-羟色胺浓度为10-5mol/L处理72h的附着率（20.73%）和变态率（20.18%）高于其他组别。因此，γ-氨基丁酸可作为羊鲍育种培育过程的有效诱导剂，可以应用推广于羊鲍幼虫的培育过程乃至大规模生产养殖中。 |
| 关键词: 羊鲍 γ-氨基丁酸 5-羟色胺 存活 附着 变态 |
| DOI：10.11693/hyhz20191200261 |
| 分类号:S968.3 |
| 基金项目:海南省重点计划项目，ZDYF2016089号；中医药行业科研专项（海洋药），201207002-03号；国家高技术研究发展计划（863）项目，2012AA10A412-8号，2004AA603130号；国家海洋公益项目，201105008-7号。 |
|
| EFFECTS OF GABA, POTASSIUM CHLORIDE, AND SEROTONIN ON LARVAL SURVIVAL, SETTLEMENT, AND METAMORPHOSIS OF ABALONE HALIOTIS OVINA |
|
PU Shu-Wei1,2,3,4, LU Feng1,2,3,4, WANG Jia-Wei1,2,3,4, HUANG Bo1,2,3,4, WANG Xiao-Bing3,4, WANG Lin-Gui3,4
|
|
1.College of Marine Sciences, Hainan University, Haikou 570228, China;2.Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province, Haikou 570228, China;3.State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China;4.Key Laboratory of Tropical Biological Resources of Ministry of Education in Hainan University, Haikou 570228, China
|
| Abstract: |
| The effects of γ-aminobutyric acid (GABA), potassium chloride, and serotonin were examined to determine the possibility of promoting larval survival, settlement, and metamorphosis of Haliotis ovina. Results show that GABA was able to increase survival, settlement, and metamorphosis. Treatment with GABA at 10-4 mol/L for 72h resulted in higher settlement rate (26.27%) and metamorphosis rate (22.26%), while at 10-6 mol/L for 72h resulted in higher survival rate (30.84%). Potassium chloride could induce settlement at high concentration (10-2-10-3 mol/L) but had no effect on survival and metamorphosis. Even higher settlement rate 11.3% and 16.4% was observed in the 10-3 mol/L group treated for both 24h and 72h, respectively. Settlement and metamorphosis could be induced by Serotonin that had no effect or had inhibitory effect on larval survival. Even more higher settlement rate (20.73%) and metamorphosis rate (20.18%) were detected at serotonin concentration of 10-5 mol/L for 72h. Therefore, GABA can be used as an effective inducer in seed culture of H. ovina. It can be applied in larval cultivation process and even mass production and breeding of H. ovina. |
| Key words: Haliotis ovina γ-aminobutyric acid (GABA) serotonin survival settlement metamorphosis |