| 摘要: |
| 为明确哈维氏弧菌感染下，泥蚶（Tegillarca granosa）不同组织弧菌载量的变化规律，通过哈维氏弧菌浸泡感染泥蚶的方法，建立弧菌浓度计数标准曲线，记录攻毒水体和泥蚶组织中的哈维氏弧菌含量的动态变化及相关关系。结果显示，弧菌感染浓度为1×107CFU/mL，泥蚶血液中弧菌载量显著高于鳃、闭壳肌、外套膜和肝胰腺，各组织中的弧菌载量呈现先上升，后下降，最后维持较低水平的变化规律。持续感染6 d，泥蚶血液中弧菌载量降至102-104 CFU/mL，其他组织中弧菌载量降至100-102 CFU/mg。检测不同浓度弧菌（1×105，1×106，1×107，5×107 CFU/mL）浸泡感染后泥蚶肝胰腺的弧菌载量变化，结果显示，1 d时各感染组肝胰腺弧菌载量均较对照组显著增加（81.0-667.8倍），且与浸泡水体中弧菌浓度呈显著正相关（Spearman's ρ=0.762，P<0.001），5 d时各侵染组肝胰腺弧菌载量均较1 d明显降低，但仍然高于对照组。研究结果为泥蚶感染弧菌发病过程中，免疫识别及免疫响应机制的研究提供了参考。 |
| 关键词: 泥蚶(Tegillarca granosa) 哈维氏弧菌(Vibrio harveyi) 弧菌载量 免疫抗性 |
| DOI：10.11693/hyhz20210300074 |
| 分类号: |
| 基金项目:国家自然科学基金项目,31902358号;国家重点研发计划项目,2018YFD0901405-2号,2020YFD0900802号;浙江省重点研发计划项目,2019C02045号,2018C02039号;财政部和农业农村部:国家现代农业产业技术体系资助;温州市农业新品种选育协作组项目,2019ZX001-1号。 |
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| LOAD VARIATION OF VIBRIO HARVEYI IN THE BLOOD CLAM TEGILLARCA GRANOSA |
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YANG Qian-Yuan1,2,3,4, TENG Shuang-Shuang2,3,4, CAI Yi-Long2,3,4, XIAO Guo-Qiang1,2,3,4, CAI Xi-Li5
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1.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;2.Zhejiang Mariculture Research Institute;3.Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bioresource;4.Wenzhou Key Laboratory of Marine Biological Genetics and Breeding, Wenzhou 325005, China;5.Pingyang Agriculture and Rural Bureau of Zhejiang Province, Pingyang 325499, China
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| Abstract: |
| To understand the variation detail of bacterial load in different tissues of the blood clam Tegillarca granosa (including gill, mantle, adductor, hepatopancreas, and blood) under Vibrio harveyi challenge, a V. harveyi immersion experiment was conducted and the standard curve of V. harveyi concentration was established by turbidimetric method, from which the dynamic relationship between the bacterial growth and reproduction in seawater under Vibrio challenge in 24 h was described, the bacterial load of the blood clam tissues was calculated, and the relationship between the Vibrio load in hepatopancreas and the immersion different concentrations (1×105, 1×106, 1×107, 5×107 CFU/mL) of Vibrio in seawater was specified. The results showed that the Vibrio load in the clam blood was significantly higher than in other tissues under the concentration of 1×107 CFU/mL. The bacterial load in the tissues was increased at first, then decreased, and finally stayed at a low level. The Vibrio load in the blood was decreased to 102-104 CFU/mL, and the Vibrio load in other tissues was decreased to 100-102 CFU/mg. Under different Vibrio concentrations, the Vibrio load in the hepatopancreas increased significantly on Day 1, which was 81.0-667.8 times of that of the control. Correlation between the bacterial load and the Vibrio dose in the seawater was significantly positive (Spearman's ρ=0.762, P<0.001). The bacterial load in the hepatopancreas was obviously decreased on Day 5, which was still higher than that of the control. This study provided a reference for the immune recognition and response during pathogenesis by Vibrio infection in T. granosa. |
| Key words: Tegillarca granosa Vibrio harveyi Vibrio load immune resistance |
