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| 运输方式对马氏珠母贝(Pinctada fucata)稚贝存活率、生长、消化及抗氧化性能的影响探究 |
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李娇妮1,2, 廖秀睿1,2, 李曦1,2, 吕布1,2, 杨守国1,2,3, 臧战1,2, VASQUEZ Herbert Ely1,2, 王爱民1,2, 郑兴1,2, 顾志峰1,2
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1.海南大学海洋学院 海南海口 570228;2.海南大学 南海海洋资源利用国家重点实验室 海南海口 570228;3.海南省海洋与渔业科学院 海南海口 571126
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| 摘要: |
| 为优化马氏珠母贝苗种运输技术,提高运输效率,以马氏珠母贝(Pinctada fucata)稚贝为研究对象,分别以有水和无水两种运输方式汽运8 h条件下开展实验,比较分析其在运输前后、运输后恢复阶段的生长、消化和抗氧化性能。结果显示:有水运输后存活率达98%,无水运输97%;在恢复期第14 d,有水运输和无水运输存活率分别达97%、96%;有水运输皮质醇含量由(2 607.61±15.27)μg/L显著降低至(1 412.98±5.09)μg/L,无水运输后显著降低至(2 287.36±10.18)μg/L;在恢复期第7 d和14 d皮质醇含量均呈逐渐上升趋势;有水和无水运输后和恢复期淀粉酶活性不断上升,且有水运输酶活性高于无水运输(P<0.05),最高值为(1.43±0.05) U/mg prot;有水和无水运输后及恢复期超氧化物歧化酶酶活性在不断下降,最低下降至(16.00±0.14) U/mg prot,有水运输超氧化物歧化酶活性均高于无水运输(P<0.05);有水运输碱性磷酸酶、谷草转氨酶和谷丙转氨酶活性均上升,无水运输均下降;在恢复阶段,碱性磷酸酶活性在不断下降,谷草转氨酶和谷丙转氨酶活性在不断上升。有水和无水运输会对马氏珠母贝稚贝产生不同的影响,有水运输方式相对无水运输方式可使马氏珠母贝稚贝在后期阶段具有更好的存活效果和生长状态。 |
| 关键词: 马氏珠母贝 稚贝 有水运输 无水运输 生长 消化性能 抗氧化能力 |
| DOI：10.11693/hyhz20210800191 |
| 分类号: |
| 基金项目:海南省自然科学青年基金,320QN207号;国家自然科学基金项目,31772847号;海南大学科研启动基金,KYQD (ZR)20061号。李娇妮,硕士研究生,E-mail:956249939@qq.com |
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| THE EFFECT OF TRANSPORTATION MODE ON SURVIVAL, GROWTH, DIGESTION AND ANTIOXIDANT CAPACITY OF PINCTADA FUCATA JUVENILES |
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LI Jiao-Ni1,2, LIAO Xiu-Rui1,2, LI Xi1,2, LYU Bu1,2, YANG Shou-Guo1,2,3, ZANG Zhan1,2, VASQUEZ Herbert Ely1,2, WANG Ai-Min1,2, ZHENG Xing1,2, GU Zhi-Feng1,2
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1.Ocean College, Hainan University, Haikou 570228, China;2.State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China;3.Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, China
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| Abstract: |
| To optimize the transportation technology and efficiency of Pinctada fucata juveniles, the survival, growth, digestion, and antioxidant capacity were studied after 8-h automobile transportation with or without water and in 7~14-day recovery. Results show that after the transportation with and without water, the survival rate was 98% and 97%, and remained high after 14-day recovery at 97% and 96%, respectively. After transportation, the cortisol decreased significantly from (2 607.61±15.27) to (1 412.98±5.09)μg/L in with-water case, while decreased to (2 287.36±10.18)μg/L in without-water case. In the recovery stage, the cortisol trended to increase gradually; and the amylase activity was increased continuously, being much higher with water [max. (1.43±0.05) U/mg prot]] than that without water. The superoxide dismutase (SOD) activity decreased continuously [min. (16.00±0.14) U/mg prot], of which with-water case was higher than without water (P<0.05). The activity of alkaline phosphatase (AKP), glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) were increased in with-water case, while decreased in without-water case. In the recovering stage after transportation, AKP activity was decreased continuously, while GPT and GOT increased. Therefore, effects of transportation mode (with/without water) on growth performance of P. fucata juveniles were different. In general, the survival and growth of P. fucata juvenile by with-water transportation were better than those with no water. |
| Key words: Pinctada fucata juveniles transported with water transported without water growth digestive performance oxidation resistance |
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