| 摘要: |
| 组蛋白乙酰化酶(histone acetyltransferases,HATs)在机体发育和环境胁迫响应的调控中发挥着重要作用,但在海洋贝类中研究极少。缢蛏(Sinonovacula constricta)作为典型的滩涂双壳贝类,经常面临多种极端环境和细菌胁迫的挑战,基于全基因组和转录组数据系统分析了缢蛏HAT基因家族(ScHATs)的系统发育关系,以及它们在不同发育时期、不同环境因子和细菌胁迫下的表达模式。结果共鉴定出11个ScHATs基因,根据序列同源性分为GNAT、MYST和p300/CBP三类,每个亚家族中几乎所有的HAT都具有特定的保守结构域和保守基序。基因表达谱和qRT-PCR分析显示,ScHATs在幼虫发育前期表达量普遍高于发育后期;在氨氮胁迫下,ScKAT2B、ScKAT8、ScKAT9-2、Scp300在鳃中的表达量显著变化(P<0.05);在高温胁迫下,ScHATs在鳃和肝胰腺中表现出相似的表达模式,而Scp300在肝胰腺中的表达量显著降低(P<0.05);在副溶血弧菌胁迫下,ScHATs的表达均出现显著上调(P<0.05)。综上结果提示,ScHATs可能在缢蛏的早期发育及抗逆抗病中发挥重要作用,系统研究将为进一步理解双壳贝类HAT基因家族的进化和功能奠定理论基础。 |
| 关键词: 缢蛏 组蛋白乙酰化酶 基因家族 环境胁迫 细菌感染 基因表达 |
| DOI：10.11693/hyhz20211000239 |
| 分类号:S968.3; Q953; Q789 |
| 基金项目:浙江省农业新品种选育重大科技专项,2021C02069-7号;宁波市“科技创新2025”重大专项,2021Z114号,2019B10005号;宁波市自然科学基金,202003N4191号;国家海洋水产种质资源库课题。 |
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| GENOME-WIDE IDENTIFICATION AND EXPRESSION ANALYSIS OF THE HISTONE ACETYLTRANSFERASE (HAT) GENE FAMILY IN SINONOVACULA CONSTRICTA UNDER ENVIRONMENTAL AND BACTERIAL STRESSES |
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LIAN Jia-Ying1, LYU Li-Yuan2, YAO Han-Han1, DONG Ying-Hui1,2, LIN Zhi-Hua1,2
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1.College of Biological and Environmental Sciences, Zhejiang Wanli University, Zhejiang Key Laboratory of Efficient Utilization Technology of Aquatic Germplasm Resources, Ningbo 315100, China;2.Ninghai Institute of Mariculture Breeding and Seed Industry, Zhejiang Wanli University, Ningbo 315604, China
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| Abstract: |
| Histone acetyltransferases (HATs) controls the regulation of body growth and the response to environmental stress. However, little is known about HATs in marine mollusks at present. The razor clam Sinonovacula constricta is a typical bivalve living in tidal flats and currently faces extreme environmental challenges. To understand the evolutionary dynamics of HAT gene family in shellfish, phylogenetic relationships and gene expression patterns of HATs in S. constricta (ScHATs) at different developmental stages, environmental stresses, and bacterial infection were analyzed and compared. A total of 11 ScHAT genes identified from S. constricta genome could be divided into three subfamilies (GNAT, MYST, and p300/CBP) in sequence homology. The protein structure prediction showed that ScHAT members belonging to each subfamily were conserved in protein domains and motifs. Gene expression analysis revealed that the expression levels of ScHATs was generally higher in the early developmental stage than that in the late stage. ScKAT2B, ScKAT8, ScKAT9-2, and Scp300 were expressed differentially in gills against ammonia-N stress (P<0.05). ScHATs showed similar expression pattern in gills and hepatopancreas under thermal stress, and Scp300 were significantly downregulated in hepatopancreas. Additionally, expressions of ScHATs increased dramatically after Vibrio parahaemolyticus infection (P<0.05). Therefore, ScHATs is an important class of genes in the development and immune defense of S. constricta. This study provides a theoretical foundation for further understanding the evolution and function of HAT gene family in mollusks. |
| Key words: Sinonovacula constricta histone acetyltransferase gene family environmental stress bacterial infection gene expression |
