| 摘要: |
| 象山港位于浙江省宁波市东南部沿海,属于半封闭式海湾,是以海水养殖区和海洋牧场示范区为主的重要多功能水域,近年来生态环境问题日益突出。为研究象山港海域在早春低水温环境中细菌的多样性,通过高通量测序方法对3月份的象山港水域进行检测。沿象山港南部海湾随机选取6个取样点(G04,G06,G08,G09,G011,G012),共检出836属和2 166种细菌,隶属33个门、91纲、186目和352科。其中优势细菌类群主要包括变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)和蓝细菌门(Cyanobacteria)。变形菌门在G04中占比最高(91.45%),拟杆菌门在G09中占比最高(18.05%);而放线菌门在G12中占比最高(7.95%)。在经度较高且离海岸较远的水域,细菌丰富度也越高;同时随着取样位点纬度的增加,γ-变形菌丰度逐步增高,其中在G04中最高(72.76%),在G12中最低(29.11%)。各取样点的细菌分离培养结果也表明弧菌属丰度较高,且主要集中在G04中。利用高通量测序方法揭示了象山港早春全水域的细菌结构组成框架图,可为象山港的水产养殖和可持续发展提供参考依据。 |
| 关键词: 象山港水域 细菌分离培养 高通量测序 细菌多样性 |
| DOI：10.11693/hyhz20211000252 |
| 分类号:Q958.1;X171 |
| 基金项目:国家重点研发计划资助项目,2017YFC1404505号;海洋经济创新发展区域示范专项,甬海办[2016]71号。 |
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| STUDY ON BACTERIAL DIVERSITY IN THE XIANGSHAN HARBOR USING HIGH THROUGHPUT SEQUENCING |
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LIU Yan1, LI Zhi-En2, YUAN Yi-Ming2, ZHANG Zhen1, QIAN Peng-Yu3, YE Huan3, LIU Song-Yi1, WANG Ze1, HAN Jiao-Jiao1, ZHOU Jun1, SU Xiu-Rong1
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1.School of Marine Science, Ningbo University, Ningbo 315211, China;2.East China Sea Environmental Monitoring Center of State Oceanic Administration, Shanghai 201206, China;3.Zhejiang Zhenghegu Biotechnology Co., Ltd., Ningbo 315048, China
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| Abstract: |
| The bacterial diversity of sea area of the Xiangshan Harbor under lower water temperature in early spring was detected by the high-throughput sequencing method, from which 2 166 bacteria species were identified, belonging to 836 genera, 186 orders, 352 families, and 91 classes. The dominant bacterial groups included mainly Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutesand, and Cyanobacteria. Proteobacteria accounted for the highest proportion in station G04 (91.45%) and Bacteroides accounted for the highest proportion in G09 (18.05%). The proportion of Actinomycetes in G12 (7.95%) is the highest. In addition, as revealed in high-throughput sequencing, the richness of bacteria increased with the increase in longitude and in distance from coast, which may be affected by the human living environment. Meanwhile, the richness of γ-Proteobacteria in the sea area was 72.76% in G04 site and 29.11% in G12 site. This study provided a reference for aquaculture and sustainable development of the Xiangshan harbor. |
| Key words: Xiangshan harbor bacterial isolation and culture high-throughput sequencing community structure |
