| 摘要: |
| 皱纹盘鲍是我国重要的增养殖贝类,近年来养殖产量增长迅速,但现有养殖群体仍存在抗逆性不足、底播增养殖低效等问题。个体身份的标记技术对于皱纹盘鲍人工选育中谱系记录,以及对于底播增殖效果的精准评估等均具有重要作用。采用金属挂片和塑料黏贴标签两种标记方法,比较了标记后皱纹盘鲍生理、生长存活、标记脱落率及被敌害捕食死亡等方面的差异。研究结果表明,与塑料黏贴标签标记和无标记对照相比,金属挂片标记对皱纹盘鲍标记早期生理包括出现黏液时间、心率变化等产生显著影响,对小规格皱纹盘鲍(壳长<3 cm)造成生长下降、死亡率高等影响,而对大规格皱纹盘鲍(壳长>3 cm)标记生成60 d后长时期的生长、存活以及逃避敌害均无显著影响;塑料黏贴标签标记方法与无标记对照组相比,均未对皱纹盘鲍造成显著影响。验证了皱纹盘鲍个体水平下两种标记方法的有效性及适用性,特别是对大规格苗种标记后生长、存活等性状无显著影响。研究结果将为皱纹盘鲍人工选育以及底播增养殖等工作提供有益指导。 |
| 关键词: 皱纹盘鲍 标记方法 呼吸率 黏液 敌害 |
| DOI：10.11693/hyhz20211100275 |
| 分类号:S931 |
| 基金项目:国家重点研发计划项目,2019YFD0900801号;国家自然科学基金项目,31972790号;烟台市科技计划项目,2019XDHZ095号;财政部和农业农村部:国家现代农业产业技术体系资助,CARS-49号。 |
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| COMPARISON IN PHYSIOLOGICAL, GROWTH, AND SURVIVAL PERFORMANCE OF PACIFIC ABALONE HALIOTIS DISCUS HANNAI BY DIFFERENT TAGGING METHODS |
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ZHANG Yuan-Fan1,2, TANG Jun-Wei3, ZHANG Jia-Wen1,2, GE Hong-Xing1, WU Fu-Cun2, ZHANG Guo-Fan2
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1.Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China;2.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;3.Changdao Marine Economic Promotion Center, Yantai 265800, China
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| Abstract: |
| Pacific abalone (Haliotis discus hannai Ino) is an economically-valuable molluscan species and fishery resource in China with increasing demand for the production. However, challenges remain in the culture with low stress resistance and low efficiency in bottom culture. The individually-tagging for Pacific abalone is important in pedigree recording of selection program and the marking-release in resource enhancement. Two tagging methods, i.e., metal clipping and plastic gluing were tested to monitor and compare differences in physiology, growth and survival rate, tag retention rate, and mortality by predator of the tagged individuals. Results show that the metal clipping affected the early physiological performances for small-size ones (shell length <3 cm) in the appearance of mucus, heart rate, etc., which reduced the growth and increased the mortality. And for large -size ones (shell length >3 cm), no significant differences were shown in the long-term growth, survival from predator in 60 days after metal-clip tagging. For the plastic-glued tagging, the overall performance was similar to untagged ones’ (the control). Both individual tagging methods were effective in Pacific abalone culture, especially large-size ones. This study provides beneficial information to Pacific abalone selection program and marking-release in resource enhancement. |
| Key words: Pacific abalone Haliotis discus hannai Ino tagging respiratory rates mucus predator |
