| 摘要: |
| 茎柔鱼(Dosidicus gigas)是重要的大洋性经济头足类,其性腺组织发育滞后于个体生长,但在性腺发育开始后生殖能量积累显著。为研究茎柔鱼脂肪积累对组织能量积累的贡献,采用组织能量密度测定技术和组织脂肪萃取技术对茎柔鱼卵巢、输卵管卵子、肌肉和消化腺4个组织脱脂前后的组织能量密度、单位质量脂肪含量及脂肪能量进行测定和分析。结果显示,脱脂前各组织的能量密度以消化腺最大,为(27.34±0.85) kJ/g;脱脂后的能量密度则以输卵管卵子的最大,为(20.01±0.56) kJ/g。各组织脱脂后的能量密度值均下降显著,尤以消化腺的下降最大,达31%。消化腺的单位质量脂肪含量最高,为(413.83±96.81) mg/g,其次为输卵管卵子和卵巢,肌肉组织的最低。在生理性成熟后,卵巢组织脂肪积累较大,输卵管卵子、肌肉和消化腺等组织则维持较稳定的脂肪积累水平。4个组织的脂肪能量占比的平均值在10%~32%之间。综上研究表明,脂肪是茎柔鱼卵巢、输卵管卵子、肌肉和消化腺等组织的重要能量物质,在组织能量积累过程中起着重要的作用。 |
| 关键词: 茎柔鱼 脂肪 脂肪能量 组织能量密度 能量积累 |
| DOI：10.11693/hyhz20211100266 |
| 分类号:S931;S932;Q956 |
| 基金项目:国家自然科学基金项目,41876144号;上海市人才发展资金项目,2020107号。 |
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| LIPID ACCUMULATION AND CONTRIBUTION TO ENERGY ACCUMULATION OF FOUR TISSUES OF DOSIDICUS GIGAS |
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FENG Yi-Xuan1, SONG Wei1, LIN Dong-Ming1,2,3,4
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1.College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China;2.Key Laboratory of Sustainable Exploitation of Oceanic Fishery Resources, Ministry of Education, Shanghai 201306, China;3.National Distant-water Fisheries Engineering Research Center, Shanghai 201306, China;4.Key Laboratory of Oceanic Fisheries Exploration, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China
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| Abstract: |
| To study the contribution of lipid content to tissue energy accumulation in Dosidicus gigas, the tissue energy density, lipid-free tissue energy density, lipid content, and energy contribution of lipids of four tissues, namely ovary, oviduct eggs, mantle soma and digestive gland, were studied, for which tissue energy density determination and lipid extraction technique were applied. Results show that tissue energy density was the highest in digestive gland on average of (27.34±0.85) kJ/g, and lipid-free tissue energy density was the highest in oviduct eggs on average of (20.01±0.56) kJ/g. After lipid extraction, the tissue energy density was significantly declined in all the tissues from those of raw tissues before lipid extraction, of which the digestive gland showed the largest drop by up to 31%. The lipid content was the largest in the digestive gland on average of (413.83±96.81) mg/g, followed by the oviduct eggs [(309.51±44.36) mg/g] and ovaries [(150.64±46.65) mg/g], and the lowest in the mantle soma [(120.52±36.07) mg/g]. For each tissue, the energy accumulation contribution from lipid content accounted for an average from 10% to 32% of the total energy accumulated. Additionally, the ovaries accumulated increasingly a large amount of lipid after reaching physiologically mature stage. In contrast, the proportion of lipid content was relatively stable throughout the sexual maturation in oviduct eggs, mantle soma, and digestive gland. Therefore, lipid is an important nutrient and energy substance stored as bioenergetic material in ovaries, oviduct eggs, mantle soma, and digestive gland, and the lipid accumulation plays an important role in energy accumulation in these tissues. These lines of evidence would warrant further researches on the energy acquisition pathway of D. gigas. |
| Key words: Dosidicus gigas lipid lipid energy tissue energy density energy accumulation |
