| 摘要: |
| 为揭示赤点石斑鱼精子的超微结构及环境因子对其精子活力的影响,利用扫描电镜和透射电镜观察赤点石斑鱼精子的超微结构,设置不同梯度的温度、盐度、pH值及不同浓度的NaCl、KCl、CaCl2、MgCl2、EDTANa2溶液,探究这些因子对赤点石斑鱼精子活力的影响。结果表明:赤点石斑鱼成熟精子的结构特点是细胞核圆形或卵圆形,核内染色质致密,没有核泡(核空隙)。精子尾部细长,横切面为典型的"9+2"微管结构。温度、盐度、pH值等环境因子对精子活力的影响表明,精子活力的适宜温度范围为23~31℃,27.5℃时精子寿命最长为37min;适宜的盐度范围为15~35,盐度15时精子寿命最长为50min;适宜的pH范围为7~9,pH为9时精子的运动时间最长为33min。赤点石斑鱼精子在EDTANa2溶液中呈抑制状态,在400~700 mmol/L的NaCl溶液、400~600mmol/L的KCl溶液和500 mmol/L的CaCl2溶液中精子均具有较好的活动能力。在MgCl2溶液中赤点石斑鱼精子的活动能力不佳。赤点石斑鱼精子的最适温度范围与繁殖季节的最适水温范围符合,适宜盐度范围较广,对pH值的变化适应性较强。赤点石斑鱼精子在NaCl、KCl、CaCl2溶液中活力较佳,在MgCl2溶液和EDTANa2溶液中呈抑制状态。 |
| 关键词: 赤点石斑鱼 精子 超微结构 精子活力 环境因子 |
| DOI：10.11693/hyhz20220300079 |
| 分类号: |
| 基金项目:国家自然科学基金项目,31972768号;广东省基础与应用基础区域联合基金,2019B1515120064号;广东省科技计划项目,2019B030316022号;中国-东盟海上合作基金资助,CAMC-2018F号;2021年国家级大学生创新创业训练计划项目,202111078034号。 |
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| ULTRASTRUCTURE OF SPERMATOZOA AND EFFECTS OF ENVIRONMENTAL FACTORS ON SPERMATOZOA MOTILITY OF GROUPER EPINEPHELUS AKAARA |
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ZHU Ya-Ting1, ZHANG Ming-Qing1, WU Yu-Xin1, ZHAO Hui-Hong2, ZHANG Hai-Fa3, SHU Hu1
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1.School of Life Sciences, Guangzhou University, Guangzhou 510006, China;2.College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China;3.Guangdong Marine Fishery Experiment Center, Huizhou 516081, China
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| Abstract: |
| To reveal the ultrastructure of spermatozoa of grouper Epinephelus akaara and the influence of environmental factors on its spermatozoa motility, the ultrastructure of spermatozoa of E. akaara was observed using transmission electron microscope and scanning electron microscope. Before the observations, the effects of temperature, salinity, pH, and different concentrations of NaCl, KCl, CaCl2, MgCl2, and EDTANa2 on spermatozoa motility of E. akaara were investigated. Results show that the nuclear structure of mature spermatozoa of E. akaara is round or oval, with dense chromatin and no nuclear vacuoles. The spermatozoa tail is elongated and the transverse section is typical of "9+2" microtubule structure. The effects of environmental factors of temperature, salinity, and pH value on spermatozoa motility showed that the suitable temperature range of sperm motility was 23~31℃, and the longest lifespan was 37 min at 27.5℃. The optimum salinity range was 15~35, and the longest sperm lifespan was 50 min at salinity 15. The optimal pH range was 7~9, and the maximum motility time was 33 min at pH 9. The spermatozoa of E. akaara showed inhibition in EDTANa2 solution, and showed good motility in 400~700 mmol/L NaCl solution, 400~600 mmol/L KCl solution, and 500 mmol/L CaCl2 solution. In the solution of MgCl2, the motility of spermatozoa was not so good. The optimum temperature range of spermatozoa of E. akaara was consistent with the optimum water temperature range in the breeding season. The suitable salinity range of spermatozoa was wide, and its adaptability to the change of pH value was strong, which accords with the characteristics of marine fishes. The spermatozoa motility was comparatively good in NaCl, KCl, and CaCl2 solutions, but inhibited in MgCl2 and EDTANa2 solutions. |
| Key words: Epinephelus akaara spermatozoa ultrastructure spermatozoa motility environmental factors |
