| 摘要: |
| 夏季高温对虹鳟(Oncorhynchus mykiss)网箱养殖会产生极大威胁,高温胁迫会造成虹鳟应激甚至死亡。为探究高温胁迫对虹鳟肝脏抗氧化响应及相关免疫基因的影响,选取虹鳟“水科一号”幼鱼[(24.8±10.0)g]为实验对象,通过连续升温达到高温胁迫条件,筛选出高温敏感组和耐受组,并对它们的抗氧化酶活性、免疫相关基因表达和随机扩增多态性DNA(RAPD)片段化进行比较研究。结果表明:敏感组与耐受组的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性含量不存在显著性差异(P>0.05),但均显著高于对照组(P<0.05);敏感组的缺氧诱导因子-1α(hif-1α)、白细胞介素-1β(il-1β)以及热休克蛋白10(hsp10)基因的相对表达量较耐受组的分别高1.8倍、1.28倍和16.5倍(P<0.05);耐受组的热休克蛋白70(hsp70)基因的表达比敏感个体高1.37倍(P<0.05);RAPD图谱反映出敏感组的DNA损伤比耐受组更严重。高温胁迫对虹鳟幼鱼hif-1α、hsp70和hsp10基因产生强烈的应答性表达上调,且RAPD针对高温耐受个体可有效进行识别。 |
| 关键词: 虹鳟 高温胁迫 缺氧诱导因子-1α 热休克蛋白 RAPD |
| DOI：10.11693/hyhz20220300064 |
| 分类号: |
| 基金项目:中央级公益性科研院所基本科研业务费专项,HSY202010Q号;财政部和农业农村部:国家现代农业产业技术体系资助,CARS-46号;中国水产科学研究院科研业务费项目,2020TD32号;烟台开发区创新创业团队项目,2021TD003号。 |
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| EFFECT OF THERMAL STRESS ON ACTIVITY OF ANTIOXIDANT ENZYMES AND EXPRESSION OF IMMUNE-RELATED GENES IN LIVER OF RAINBOW TROUT (ONCORHYNCHUS MYKISS） |
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DONG Fu-Lin1,2, HUANG Tian-Qing1, LIU En-Hui1, GU Wei1, WANG Gao-Chao1, GUO Fu-Yuan3, WANG Bing-Qian1, XU Ge-Feng1
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1.Cold Water Fish Industry Technology Innovation Strategic Alliance, Ministry of Agriculture and Rural Affairs, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;2.Shanghai Ocean University, Shanghai 201306, China;3.Yantai Jinghai Marine Fishery Co. Ltd., Yantai 264006, China
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| Abstract: |
| High summer temperatures poses a threat to rainbow trout Oncorhynchus mykiss in net pens, causing thermal stress or even death. To investigate the effects of thermal stress on the antioxidant response of rainbow trout liver and related immune genes, we selected juvenile rainbow trout [(24.8±10.0) g] as the experimental subjects and screened the sensitive and tolerant groups by gradual temperature increases to a thermal stress point. Polymorphic DNA (RAPD) fragmentation was studied in a comparative manner. Results show that the levels of superoxide dismutase (SOD) and catalase (CAT) activities in the sensitive group showed no significant difference from those in the tolerant group (P>0.05) but the control group (P<0.05). The levels of hypoxia inducible factor 1α (hif-1α), interleukin-1β (il-1β), and heat shock proteins10 (hsp10) in the sensitive group had no significant difference from those in the tolerant group (P<0.05). The relative expression of hif-1α, il-1β, and hsp10 were 1.8, 1.28, and 16.5 times higher in the sensitive group than in the tolerant group (P<0.05), respectively. The expression of heat shock proteins 70 (hsp70) in the tolerant group was 1.37 times higher of that in the sensitive group (P<0.05). The expression of il-8 and hsp10 in the sensitive group was significantly higher than that in the tolerant group, indicating that the high temperature led to an inflammatory response in the liver of juvenile rainbow trout, and the liver of the sensitive group was more severely damaged, which is consistent with the RAPD profile reflecting that the DNA damage in the sensitive group was more severe than that in the tolerant group. The expression of hsp70 in the tolerant group was significantly higher than that in the sensitive group because hsp70 plays a role in protecting tissues and cells in rainbow trout in response to the heat stress. It is suggested that the difference in hsp70 and hsp10 expression is one of the reasons for the difference in thermal tolerance and the ability to tolerate hypoxia is an important factor in thermal tolerance for rainbow trout. Meanwhile, the RAPD is an effective tool for identifying individuals with thermal tolerance. |
| Key words: rainbow trout Oncorhynchus mykiss thermal stress hypoxia-inducible factor-1α heat shock protein RAPD |
