| 摘要: |
| 全球气候变化导致海洋氧含量降低, 海水低氧胁迫对海洋无脊椎动物的生长、发育和繁殖造成了严重的威胁。以海洋线虫Litoditis marina为实验对象, 观察了其在不同氧浓度(21%、3%、1% 和 0.5%)条件下的生长发育速率, 并对不同氧浓度条件下的L1幼虫样品进行了转录组测序和分析。实验结果表明,当环境氧浓度从21%下降至3%时, L. marina的发育成熟速度明显加快, 进一步下降至1%时, 产卵时间延长并和21%氧浓度接近, 但当氧浓度为0.5%时, L. marina的产卵时间显著延迟。比较转录组分析表明, 相比于21%氧浓度环境, 3%、1%和0.5%低氧条件下L. marina的糖酵解、糖异生、硫代谢和线粒体碳代谢等通路相关基因的表达显著上调; 而寿命调控通路、细胞色素P450代谢通路和ABC转运蛋白相关基因的表达显著下调。研究结果发现的海洋线虫应对氧浓度胁迫的基因表达变化模式, 为深入理解海洋无脊椎动物应答低氧胁迫分子机制提供了重要参考。 |
| 关键词: 海洋线虫 Litoditis marina 低氧胁迫 糖酵解 硫代谢 碳代谢通路 |
| DOI：10.11693/hyhz20220900225 |
| 分类号:Q344 |
| 基金项目:国家重点研发项目,2018YFD0901301号；青岛创业创新领军人才项目,Grant16-8-3-19-zhc号；中国科学院海洋大科学研究中心重点部署项目,2019.11~2022.11 |
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| TRANSCRIPTOME ANALYSIS OF THE RESPONSE OF MARINE NEMATODE LITODITIS MARINA TO HYPOXIA STRESS |
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LI Bo1,2,3,4, XIE Yu-Su1,2,3, ZHANG Liu-Suo1,2,3
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1.CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;4.University of Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| Global climate change has led to ocean deoxygenation, and the hypoxic stress poses a serious threat to the growth, development and reproduction of marine invertebrates. In this study, the marine nematode Litoditis marina was exploited to analyze the impact of hypoxia to marine invertebrates. The L. marina L1 larvae were sampled for transcriptome sequencing and analysis and the growth and development rate under different oxygen concentrations (21%, 3%, 1% and 0.5%) were observed. Results showed that when ambient oxygen concentration decreased from 21% to 3%, the egg-laying time of L. marina was significantly accelerated, and when it further decreased to 1%, the egg laying time of L. marina was similar to that in 21% oxygen. When the oxygen concentration dropped to 0.5%, the egg laying time was significantly attenuated. The KEGG enrichment analysis showed that genes in several pathways such as glycolysis, gluconeogenesis, sulfur metabolism, and mitochondrial carbon metabolism were significantly up-regulated in 3%, 1% and 0.5% oxygen conditions, compared to those in 21% oxygen. However, the expression levels of genes in certain pathways such as nematode lifespan regulation, cytochrome P450 metabolism, and ABC transporters were significantly down-regulated under hypoxia conditions. This study provided a foundation to further study the functions of key genes in response or adaptation to hypoxia stress, in the context of global climate change and ocean deoxygenation. |
| Key words: marine nematode Litoditis marina hypoxia stress glycolysis sulfur metabolism carbon metabolism pathway |
