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| 不同氮源、磷源对链状裸甲藻生长与酶活性的影响 |
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史竞园1,2, 刘云1,3, 张樱馨1,2, 张绪涛1,2, 宋书群1,3, 李才文1,3,2,4
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1.中国科学院海洋研究所 中国科学院海洋生态与环境科学重点实验室 山东青岛 266071;2.中国科学院大学 北京 100049;3.崂山实验室 海洋生态与环境科学功能实验室 山东青岛 266237;4.中国科学院海洋大科学研究中心 山东青岛 266071
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| 摘要: |
| 为揭示链状裸甲藻(Gymnodinium catenatum)对不同氮源和磷源营养条件的适应机制, 通过在不同营养盐浓度和形态条件下对链状裸甲藻进行一次性培养, 探究了氮源、磷源对链状裸甲藻生长和酶活性的影响。结果显示, 链状裸甲藻可在多种形态氮和磷的条件中生存, 其中链状裸甲藻在氮浓度0~800 μmol/L范围内对NH4Cl的亲和性最高, 在磷浓度0~32 μmol/L范围内对三磷酸腺苷(ATP)的利用能力最低。不同氮形态处理组中, 培养初期谷氨酰胺合成酶在NH4Cl为氮源的条件下活性表达最强; 培养中后期, 谷氨酰胺合成酶和脲酶在尿素为氮源条件中表达最高, 而各处理组中的硝酸还原酶活性均较低, 表明链状裸甲藻在低硝酸盐环境中没有竞争优势。不同磷形态处理中, 各组碱性磷酸酶活性随培养时间先升高后降低, 酸性磷酸酶活性(除ATP处理组外)逐渐降低。ATP为磷源的处理组具有最高的碱性磷酸酶和酸性磷酸酶活性, 其他三个处理组酶活性表达相似。研究发现不同氮源和磷源显著影响链状裸甲藻的生长, 藻细胞可通过调节谷氨酰胺合成酶、脲酶、碱性磷酸酶和酸性磷酸酶的活性对不同形态营养盐进行利用, 研究结果为阐释链状裸甲藻对复杂营养条件的适应机制及其赤潮发生机制提供了重要参考。 |
| 关键词: 链状裸甲藻 氮源 磷源 藻生长 酶活性 |
| DOI：10.11693/hyhz20221200334 |
| 分类号:X55 |
| 基金项目:国家自然科学基金, 41976136号;国家重点研发项目, 2022YFC3105202。 |
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| EFFECTS OF NITROGEN AND PHOSPHORUS SOURCES ON GROWTH AND ASSIMILATION ENZYMES OF DINOFLAGELLATE GYMNODINIUM CATENATUM |
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SHI Jing-Yuan1,2, LIU Yun1,3, ZHANG Ying-Xin1,2, ZHANG Xu-Tao1,2, SONG Shu-Qun1,3, LI Cai-Wen1,3,2,4
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1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Marine Ecology and Environmental Science laboratory, Laoshan Laboratory, Qingdao 266237, China;4.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
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| Abstract: |
| To classify the adaption mechanism of Gymnodinium catenatum to nitrogen and phosphorus, a batch culture of G. catenatum was carried out in different sources (species) of nitrogen and phosphorus. G. catenatum was able to grow well on multiple N and P substrates within appropriate ranges of concentrations. The dinoflagellate showed the greatest affinity to NH4+ in the nitrogen concentration of 0~800 μmol/L, while the lowest capacity for utilizing ATP (adenosine triphosphate) in the phosphorus concentration of 0~32 μmol/L. At the early stage of the growth, the glutamine synthetase activity was the highest when grown on NH4+. Along with the growth of G. catenatum, both glutamine synthetase and urease were induced by urea. Additionally, the activity of nitrate reductase was extremely low, indicating its competitive weakness in the nitrate-deplete environment. In all P treatments, the alkaline phosphatase activity increased with time and then decreased, while the acid phosphatase activity (except ATP treatment) decreased. Both enzymes expressed the highest activity when grown on ATP and showed similar activity among other three P sources. Results indicate that different nitrogen and phosphorus species had significant effects on the growth of G. catenatum. The dinoflagellate could regulate the activity of glutamine synthetase, urease, alkaline phosphatase, and acid phosphatase to assimilate different types of nutrients. This study provided a reference for revealing the adaptation mechanism of G. catenatum to different nutrients, and promoted fundamental knowledge regarding the formation mechanism of G. catenatum blooms along the coast of China. |
| Key words: Gymnodinium catenatum nitrogen phosphorus growth enzyme activity |