首页 | 期刊介绍 | 编委会 | 道德声明 | 投稿指南 | 常用下载 | 过刊浏览 | In English
引用本文:王 荣.荧光法测定浮游植物色素计算公式的修正[J].海洋科学,1986,10(3):1-5.
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 1696次   下载 1570 本文二维码信息
码上扫一扫!
分享到: 微信 更多
荧光法测定浮游植物色素计算公式的修正
王 荣1
中国科学院海洋研究所
摘要:
关键词:  
DOI:
分类号:
基金项目:
CORRECTION OF THE FORMULA TO CONVERT FLUORESCENCE MEASUREMENT TO PHAEOPHORBIDE a CONCENTRATION IN ACIDIFICATION METHOD
Abstract:
In the acidification method for measuring chlorophyll a and phaeophorbide a the fluorescence readings are converted to pigments concentration using the formulae originally given by Lorenzen (1966), which were rewritten in more simple and convenient form by Strickland and Parsons (1972). They are as follows......These two formulae have been widely used for two decades in biological oceanography studies. As we know now that phaeophorbide a is almost the only degradation product present, which is abundant in water column and in herbivores fecal pellets. Recently we found, by rederivation, that Lorenzen’s second formula gives not the weight concentration of phaeophorbide a but its equivalent (on molar basis) weight concentration of chlorophyll a. So the equation must be corrected by a factor of 0.6633, the ratio of molecular weight of phaeophorbide a to that of chlorophyll a. This means that all the phaeopigment data obtained earlier by this method are 50.8% higher than their real value. Though the error is systematic, it is too big that some wrong conception and wrong conclusion can be derived. Based on their seriously deviated data Shuman and Lorenzen (1975) concluded that the conversion of chlorophyll a to phaeophorbide a after the digestion of herbivorous copepod was 100% on a molar basis. In fact their experiments showed a pigments loss as high as 34%. Furthermore, based on this wrong conclusion, plant pigments were used as tracers to study assimilation efficiency in copepods by some zooplanktologist. Our grazing experiments showed a pigments loss ranged from 6% to 80%, which is closely related with the digestion efficiency. When food was abundant in environment, ingestion rate and gut contents were high, the pigments loss would be small, and vice versa. It seems true that some of the pigments can be destroyed or absorbed during the passage through the gut of herbivores.
Key words:  
版权所有 《海洋科学》 Copyright©2008 All Rights Reserved
主管单位:中国科学院 主办单位:中国科学院海洋研究所
地址:青岛市市南区福山路32号  邮编:266071  电话:0532-82898755  E-mail:marinesciences@qdio.ac.cn
技术支持:北京勤云科技发展有限公司