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| 引用本文: | 田治立,王长海,于 贞,金海珠.纤细角毛藻培养条件优化[J].海洋科学,2005,29(2):5-7. |
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| 纤细角毛藻培养条件优化 |
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田治立1, 王长海1, 于 贞2, 金海珠2
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1.大连理工大学生物工程系;2.烟台大学海洋生化工程研究所
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| 摘要: |
| 采用正交实验的方法 ,对影响纤细角毛藻(Chaetoceros gracilis)生长的主要营养因素进行了优化 ,获得了以海水为基础的优化培养基配方: NaHCO3 0.15g/L, Na2SiO3(9H2 O0.20g/L, NaNO3 1.00g/L, KH2PO4 0.02g/L, VB1 2.7mg/L, VB12 1.5μg/L。实验表明 ,用该优化配方培养纤细角毛藻具有生长速度快、产量高的显著优势 ,为该微藻在光生物反应器中的进一步高密度培养提供了良好条件。 |
| 关键词: 纤细角毛藻(Chaetoceros gracilis) 培养 优化 |
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| Optimization of culture condition for Chaetoceros gracilis |
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| Abstract: |
| Major nutrient factors were optimized by means of orthogonal tests. The optimum medium was obtained NaHCO3 0. 15g / L, Na2SiO3·9H2O 0. 20 g / L, NaNO3 1. 00g / L, KH2PO4 0. 02 g / L, VB1 2. 7 mg / L, VB12 1. 5μg / L, which evidently increased the growth speed and cell biomass productivity of Chaetoceros gracilis compared to well - known medium f / 2. The new redia established a basis for high density culture of Chaetoceros gracilis in airlift loop photobioreactor. |
| Key words: Chaetoceros gracilis culture optimization |
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