引用本文:	姚继承,王诚,左正宏,林芳,陈奕欣.⁶⁰Co－γ辐照对坛紫菜原生质体诱变的研究[J].海洋科学,2005,29(11):48-51.

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⁶⁰Co－γ辐照对坛紫菜原生质体诱变的研究
姚继承¹, 王诚¹, 左正宏¹, 林芳¹, 陈奕欣¹
厦门大学生命科学学院

摘要:

利用⁶⁰Co-γ射线对坛紫菜(Porphyra haitanensis)原生质体进行辐照诱变,发现原生质体对⁶⁰Co-γ射线有极强的耐受性。前期的培养结果表明，原生质体是一种理想的诱变材料。诱变结果表明，⁶⁰Co-γ射线对坛紫菜可诱变剂量范围很大，从200～1 300 Gy 均有突变株生成。诱变后细胞的存活率不宜作为坛紫菜诱变最佳剂量的指标。

关键词: 坛紫菜(Porphyra haitanensis) 原生质体 ⁶⁰Co-γ射线诱变

DOI：

分类号:

基金项目:国家863 计划项目（2002AA603023）；福建省重大农业科技项目（2001Z017）

Mutagenesis on protoplast of Porphyra haitanensis by ⁶⁰Co-γ

Abstract:

⁶⁰Co-γ ray was used to induce mutant on protoplast of Porphyra haitanensis. The results showed that the protoplasts of P. haitanensis could survive under a wide range of γ-rays. Primary culture showed that the protoplast was suitable for mutagenesis. Further results also showed that mutants could be induced in a very wide range of dosages of ⁶⁰Co-γ ray. The mortality was not a suitable parameter for the evaluation of the mutant-induction efficiency in P. haitanensis.

Key words: Porphyra haitanensis protoplast ⁶⁰Co –γ ray mutagenesis