引用本文:	韩兴梅,李元广,魏晓东,李兴武,孙勇如,王义琴.转兔防御素基因小球藻培养的营养模式研究[J].海洋科学,2006,30(7):48-52.

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转兔防御素基因小球藻培养的营养模式研究
韩兴梅¹, 李元广¹, 魏晓东¹, 李兴武¹, 孙勇如², 王义琴²
1.华东理工大学海洋生化工程研究所生物反应器工程国家重点实验室;2.中国科学院遗传与发育生物学研究所

摘要:

在250mL摇瓶中研究了光自养、异养、混合营养3种营养模式对转兔防御素基因(NP-1)小球藻(Chlorella)的生长、NP-1表达量、总蛋白质含量、pH、葡萄糖消耗以及光衰减等几个方面的影响。结果表明,异养培养为转NP-1基因小球藻的最适营养模式。在此基础上,采用5,15,30L生物反应器补料分批异养培养转NP-1基因小球藻,细胞质量浓度分别达到3.67,4.87,6.39g/L。

关键词: 转基因小球藻兔防御素营养模式表达

DOI：

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基金项目:国家863计划海洋生物技术主题资助项目(2002AA629110)

Effect of culture modes on the growth of transgenic Chlorella and rabbit defensin expression

Abstract:

The influences of culture modes including autotrophy, heterotrophy, mixotrophy on process characteristics of transgenic Chlorella with rabbit defensin (NP-1) gene in the 250mL flasks were investigated including cell growth, NP-1expression, protein contents, pH, glucose consumption and light attenuation. The results showed that heterotrophy was the optimal culture mode for the transgenic Chlorella with NP-1 gene. Based on these results, transgenic Chlorella was cultured in bioreactors of 5, 15, 30L and the corresponding cell densities were 3.67, 4.87, 6.39g/L, respectively.

Key words: Transgenic Chlorella rabbit defensin culture modes expression