| 摘要: |
| 研究了不同浓度的抗冻剂二甲基亚砜(DMSO)和不同保存时 间对真鲷(Pagrus major)冻精质量的影响。试验结果表明,DMSO体积分数和保存时间对冻精的受精率、孵化率影响显著。15%二甲基亚砜作为抗冻剂保存10～60 d的冻精受精率、孵化率与鲜精无差异,而保存360 d的冻精受精率、孵化率开始出现下降。12%,18%,21%DMSO保存60 d的冻精受精率差异不显著,均高于90%,而对于保存360 d的冻精受精率、孵化率均显著地降低。 |
| 关键词: 真鲷(Pagrus major) 超低温保存 抗冻剂浓度 二甲基亚砜(DMSO) 保存时间 受精力 |
| DOI： |
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| 基金项目:国家863计划项目(2001AA621100,2003AA603510,2004AA603310) |
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| The effect of storage time and cryoprotectant concentration on sperm quality of red seabream(Pagrus major) |
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| Abstract: |
| The present study focused on the effects of the storage time and cryoprotectant concentration on the post-thaw sperm quality in red seabream(Pagrus major). The results demonstrate that cryoprotectant concentration and storage time could significantly influence the fertilization and hatching rates of post-thaw sperm. The fertilization capability of the post-thaw sperm cryopreserved with 15% DMSO for 360 d began to decrease;however, no significant difference was found among 10～60 d cryopreserved sperm. No significant differences were found among 12%～21% DMSO cryoopreserved sperm for 60 d in fertilization capacity, and the fertilization rate was higher than 90%; however, the fertilization capacities of 12%, 18%, 21% DMSO cryopreserved for 360 d were reduced significantly. The effects of the storage time, and cryoprotectant concentration, and their interaction still need further study. |
| Key words: red seabream(Pagrus major) cryopreservation cryoprotectant concentration DMSO storage time fertilization capacity |
