| 摘要: |
| 在不同海水温度和不同精子密度条件下对四倍体(4n) 与二倍体(2n) 太平洋牡蛎(Crassostrea gigas Thunberg)离体精子的存活能力进行了比较。结果表明, 四倍体与二倍体太平洋牡蛎精子的存活时间均随水温的升高和密度的降低而缩短, 四倍体牡蛎精子的存活能力低于二倍体。四倍体牡蛎精子在海水温度10℃可存活8h, 在28℃仅存活2h; 二倍体牡蛎精子在10℃可存活12h, 在28℃仅存活4h。四倍体牡蛎精子在较高密度(6.4×107个/mL)下可存活8h, 较低密度(2.0×106个/mL)下低于1h; 在与四倍体牡蛎精子相同的高、低密度下, 二倍体存活时间分别超过10h 和1h。 |
| 关键词: 太平洋牡蛎(Crassostrea gigas Thunberg) 四倍体 二倍体 精子 存活 |
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| 基金项目:国家自然科学基金项目(30771662); 国家863计划项目( 2006AA10A401); 国家科技基础条件平台项目(2005DKA30470) |
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| Comparison on survival time of spermatozoon between diploid and tetraploid Pacific Oysters, Crassostrea gigas Thunberg |
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| Abstract: |
| The survival capacity of sperm from diploid (2n) and tetraploid (4n) Pacific Oysters, Crassostrea gigas in different temperatures and densities was compared in this study. The results showed that survival time of sperm from both 2n and 4n shortened with the increased temperatures and reduced densities. The survival capacity of sperm from 4n was lower than that from 2n. Sperm from 4n could survive 8h at 10℃ and 2h at 28℃, respectively, but the sperm from 2n can survive 12h at 10℃ and 4h at 28℃, respectively, but only 8h and 2h could the sperm from 4n survive at both temperatures. For the sperm from 4n, the survival time was about 8h in high density(6.4×107 cells/mL) and less than 1h in low density( 2.0×106 cells/mL). However the sperm from 2n could survive more than 10h in high density and more than 1h in low density. |
| Key words: Crassostrea gigas tetraploid diploid sperm survival |
