| 引用本文: | 王连芬,庞广昌,米小媛.螺旋藻蛋白水解物对小鼠血清中细胞因子的影响[J].海洋科学,2008,32(10):51-55. |
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| 螺旋藻蛋白水解物对小鼠血清中细胞因子的影响 |
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王连芬1, 庞广昌1, 米小媛1
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天津市食品生物技术重点实验室, 天津商业大学生物技术与食品科学学院
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| 摘要: |
| 为了探索螺旋藻(Spirulina)蛋白及其水解物对Balb/c小鼠血清中IL-10,TGF-α,IL-4,IL-6,TNF-α,IFN-γ,IL-1β细胞因子的影响,采用盐析法从螺旋藻中提取可溶性螺旋藻蛋白,以胰蛋白酶水解得到螺旋藻蛋白水解物溶液。将所得水解物对4组Balb/c小鼠分别进行灌胃(两个灌胃组,其中一组灌胃螺旋藻干粉)、腹腔注射、尾静脉注射,用ELISA试剂盒测定了各组小鼠血清中IL-10,TGF-α,IL-4,IL-6,TNF-α,IFN-γ,IL- 1β细胞因子变化情况。结果表明,与相应空白组相比,每组中绝大部分细胞因子水平均有所提高,仅有个别给药方式的部分细胞因子有所降低(尾静脉注射的IL-10,腹腔注射的IL-4,灌胃组的TNF-α和IL-1β);螺旋藻干粉灌胃组与螺旋藻蛋白水解物灌胃组结果显示,细胞因子浓度都很低,且两组间相差也不大;3种给药方式中,腹腔注射的作用效果最为明显,尤以该注射下的IL-6浓度变化极为显著;腹腔注射的IL-10,以及尾静脉注射下的IL-6、IL-1β和IFN-γ也相当显著,说明螺旋藻蛋白水解物通过对细胞因子水平的调节.进而对机体免疫功能发挥重要的调节作用。从给药途径角度,腹腔注射效果最为突出,说明螺旋藻蛋白主要是通过肠黏膜发挥作用,设法直接通过肠黏膜途径给药可能是一种最有效的给药方式。 |
| 关键词: 螺旋藻蛋白 细胞因子 免疫调节 |
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| 基金项目:天津市重点自然科学基金项目(06YFGZNC04200) |
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| The effect of Spirulina protein hydrolysates on cytokines in the serum of mice |
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| Abstract: |
| In order to explore the effect of Spirulina protein and its hydrolysates on IL-10, TGF-α, IL-4, IL-6, TNF-α, IFN-γ, IL-1βcytokines which are in the serum of Balb/c mice, Spirulina proteins were derived from Spirulina with salting out. Then they were hydrolysated with trypsin for protein hydrolysates solution. Hydrolysis was respectively treated for mices with four ways: gavage (two groups of gavage, one group was given by Spirulina powder), intraperitoneal injection, intravenous injection, then we measured IL-10, TGF-α, IL-4, IL-6, TNF-α, IFN-γ, IL-1βchanges with ELISA kits. The results are as follows: compared with relative normal saline group, most of the eytokines have increased, only some of the individual cytokines with injection methods were reduced (IL-10 in the tail vein injection, IL-4 in the intraperitoneal injection, TNF-αand IL-1βin the gavage group); the affects of the Spirulina powder garage group and Spirulina protein garage group have no much difference; Among three administration methods, the most obvious treatment was intraperitoneal injection, especially which of IL-6 was the most signifiable, IL-6 in the tail vein injection, IL-10 in the intraperitoneal injection, in addition, IL-6, IL-1βand IFN-γin the tail vein injection were more signifiable. It was suggested that Spirulina protein hydrolysates played an important regulatory role for body immune function by regulating cytokines levels. From the point of drug deliveries, the effect of intraperitoneal injection was the most markable, and managing to deliver drug directly by intestinal mucosal is possibly a kind of the most effective drug delivery. So Spirulina protein hydrolysates are available to the development of the potential drug. |
| Key words: Spirulina protein cytokine immune regulation |
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