| 摘要: |
| 研究了黑鲷 (Sparusmacro cephalus) 在实验生态条件下暴露于不同浓度苯并(a)芘 (benzo(a)pyrene, BaP)后，肝脏乙氧基异吩噁唑酮脱乙基酶 (EROD)活性在不同暴露时相的动力学变化情况。EROD活性采用动力学方法测定。实验结果表明,暴露于较低质量浓度BaP (0.5,1.0 μg/L)黑鲷肝脏的EROD活性在2 d后极显著高于对照组(P<0.01)， 2.0 μg/L组则在2 h后极显著高于对照组，并且随暴露时间的延长,诱导程度表现出不同的下降趋势，而暴露于高质量浓度组(5.0 μg/L) 的肝脏EROD活性极显著性诱导出现在12 h。总体上较高质量浓度组(2.0,5.0μg/L) EROD活性出现极显著性诱导的时间比低质量浓度组(0.5,1.0μg/L)早，诱导程度也比低质量浓度组的高。在净化实验中各暴露组肝脏EROD活性均下降，与空白对照组比较均没有显著性差异，说明试验浓度没有超出严重损伤肝脏自身恢复系统的范围，在此浓度范围内污染的鱼通过净化可清除BaP污染。 |
| 关键词: 苯并(a)芘 乙氧基异吩噁唑酮脱乙基酶（EROD） 黑鲷 (Sparusmacro cephalus) 肝脏 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目(40106012 ) |
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| Study on the kinetics of hepatic EROD activities in black porgy(Sparusmacro cephalus) exposed to benzo(a)pyrene |
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| Abstract: |
| Laboratory experiments were carried out to study the kinetic processes of the activities of EROD (7- ethoxyresorufin O-deethylase) in the liver of Bacsilewsky exposed to benzo(a)pyrene at the different concentrations during 14 days’s exposure. Hepatic EROD activities were assayed by a slight modification of the method described by Hodson and Kirby. Our Experiments found that the strong induction of EROD activity exposure to the relatively higher concentration groups, appeared earlier than that of exposure to the relatively lower concentration groups. Results showed that the hepatic EROD activities were strong significantly induced at the second day’s exposure to the lower concentrations of benzo[a]pyrene(0.5μg/L and 1.0μg/L ), at 12h exposure to the higher concentrations of benzo[a]pyrene(2.0μg/L and 5.0μg/L ) ,compared to the blank control. The activities of hepatic EROD decreased under the different exposure levels after7 days cleaned in the sand filtered seawater and had not significant statistic difference compared with the blank control group. This indicats that BaP at our experimental concentrations did not damage the system of hepatic restored of Bacsilewsky. |
| Key words: benzo(a)pyrene EROD Sparusmacro cephalus,liver |
