引用本文:	程宁宁,杨爱国,刘志鸿,周丽青,吴彪.栉孔扇贝(♀)×虾夷扇贝(♂)子一代杂种优势的SRAP分析[J].海洋科学,2009,33(10):107-111.

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栉孔扇贝(♀)×虾夷扇贝(♂)子一代杂种优势的SRAP分析
程宁宁^1,2, 杨爱国¹, 刘志鸿¹, 周丽青¹, 吴彪¹
1.中国水产科学院黄海水产研究所;2.上海海洋大学

摘要:

采用新型的分子标记技术(Sequence-related amplified polymorphism，简称SRAP)对栉孔扇贝(Chlamys farreri)(♀)×虾夷扇贝(Patinopecten yessoensis)(♂)子一代及其双亲的遗传变异进行分析。从不同的引物组合中筛选出9对条带清晰、多态性丰富的组合，9对引物共产生了121条扩增带，其中多态性条带107条，平均每对引物组合产生11.89条多态标记。Shannon信息指数I分别为0.299 9、0.218 9、0.334 3，Nei’s基因多样性指数h分别是0.196 4、0.141 1、0.218 9，栉孔扇贝与杂交后代的遗传距离是0.107 4，虾夷扇贝与杂交后代的遗传距离是0.228 7，聚类分析也显示了这一特点。从变异贡献率来看，3个群体的总变异中，有37.31%的变异来源于群体间，62.69%的变异来源于个体间。

关键词: 栉孔扇贝(Chlamys farreri) 虾夷扇贝(Patinopecten yessoensis) 杂种优势 SRAP

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基金项目:国家863计划资助项目(2006AA10A408);国家科技支撑计划资助项目(2006BAD01A13-6);国家科技基础条件平台建设资助项目(2006DKA30470-006)

Analysis on heterosis of Chlamys farreri × Patinopecten yessoensis by SRAP marker

CHENG Ning-ning,YANG Ai-guo,LIU Zhi-hong,ZHOU Li-qing,WU Biao

Abstract:

Sequence-related amplified polymorphism(SRAP) markers were used to examine genetic variations of two parents Chlamys farreri(♀), Patinopecten yessoensis(♂) and their hybrids (F1). 9 pairs of primer were selected from 64 pairs of primer and applied to 3 populations. A total of 121 bands were generated with 9 pairs of primers sets, among them 107 were polymorphic. In average, each primer set deteced 11.89 polymorphic loci. Shannon information indexes were 0.299 9, 0.218 9, 0.334 3 and Nei's gene diversities were 0.196 4, 0.141 1, 0.218 9.The genetic distance between C. farreri and F1 was 0.107 4, between P. yessoensis and F1 was 0.228 7.

Key words: Chlamys farreri Patinopecten yessoensis heterosis SRAP