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| 引用本文: | 程宁宁,杨爱国,刘志鸿,周丽青,吴 彪.栉孔扇贝(♀)×虾夷扇贝(♂)子一代杂种优势的SRAP分析[J].海洋科学,2009,33(10):107-111. |
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| 栉孔扇贝(♀)×虾夷扇贝(♂)子一代杂种优势的SRAP分析 |
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程宁宁1,2, 杨爱国1, 刘志鸿1, 周丽青1, 吴 彪1
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1.中国水产科学院黄海水产研究所;2.上海海洋大学
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| 摘要: |
| 采用新型的分子标记技术(Sequence-related amplified polymorphism，简称SRAP)对栉孔扇贝(Chlamys farreri)(♀)×虾夷扇贝(Patinopecten yessoensis)(♂)子一代及其双亲的遗传变异进行分析。从不同的引物组合中筛选出9对条带清晰、多态性丰富的组合，9对引物共产生了121条扩增带，其中多态性条带107条，平均每对引物组合产生11.89条多态标记。Shannon信息指数I分别为0.299 9、0.218 9、0.334 3，Nei’s基因多样性指数h分别是0.196 4、0.141 1、0.218 9，栉孔扇贝与杂交后代的遗传距离是0.107 4，虾夷扇贝与杂交后代的遗传距离是0.228 7，聚类分析也显示了这一特点。从变异贡献率来看，3个群体的总变异中，有37.31%的变异来源于群体间，62.69%的变异来源于个体间。
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| 关键词: 栉孔扇贝(Chlamys farreri) 虾夷扇贝(Patinopecten yessoensis) 杂种优势 SRAP |
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| 基金项目:国家863计划资助项目(2006AA10A408);国家科技支撑计划资助项目(2006BAD01A13-6);国家科技基础条件平台建设资助项目(2006DKA30470-006) |
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| Analysis on heterosis of Chlamys farreri × Patinopecten yessoensis by SRAP marker |
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CHENG Ning-ning,YANG Ai-guo,LIU Zhi-hong,ZHOU Li-qing,WU Biao
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| Abstract: |
| Sequence-related amplified polymorphism(SRAP) markers were used to examine genetic variations of two parents Chlamys farreri(♀), Patinopecten yessoensis(♂) and their hybrids (F1). 9 pairs of primer were selected from 64 pairs of primer and applied to 3 populations. A total of 121 bands were generated with 9 pairs of primers sets, among them 107 were polymorphic. In average, each primer set deteced 11.89 polymorphic loci. Shannon information indexes were 0.299 9, 0.218 9, 0.334 3 and Nei's gene diversities were 0.196 4, 0.141 1, 0.218 9.The genetic distance between C. farreri and F1 was 0.107 4, between P. yessoensis and F1 was 0.228 7.
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| Key words: Chlamys farreri Patinopecten yessoensis heterosis SRAP |
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