| 摘要: |
| 以吩嗪硫酸甲酯 NADH为超氧阴离子自由基（O·-2）产生、检测体系和EDTANa2-Fe(Ⅱ)-H2O2为羟自由基（·OH）产生、检测体系，对壳聚糖硫酸酯铜、锌配合物和不同分子质量壳聚糖进行了抗O·-2和·OH自由基的活性研究。结果表明，壳聚糖硫酸酯金属配合物对于O·-2自由基的清除能力明显高于壳聚糖，在质量浓度为0.025 g/L时，壳聚糖硫酸酯铜配合物对O·-2自由基的清除能力达到94.18%，壳聚糖硫酸酯锌配合物达到93.19%；壳聚糖硫酸酯铜、锌配合物对·OH自由基的清除能力（67.39%、60.46%）低于相同分子质量的壳聚糖（88.06%），而高于高分子质量壳聚糖761 ku(18.71%)；壳聚糖分子质量大小对O·-2和·OH自由基的清除能力有较大影响，质量浓度为1.6 g/L壳聚糖分子质量为20 ku时，对O·-2清除率达54.69％，而分子质量在761 ku时，对O·-2清除率仅为35.50％；各样品对O·-2和·OH自由基的清除能力均随着质量浓度的增加而上升，壳聚糖硫酸酯铜、锌配合物在相当低的浓度下（0.025 g/L）就可以达到明显清除O·-2自由基的效果（≥90％）。
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| 关键词: 壳聚糖 壳聚糖硫酸酯金属配合物 超氧阴离子自由基（O·-2） 羟自由基（·OH） 抗氧化活性 |
| DOI： |
| 分类号: |
| 基金项目:国家863计划重点项目（2007AA091601）;中国科学院知识创新工程项目(KZX-2-YW-209) |
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| Antioxidant activity of the complexes of sulfation chitosan with metalion |
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LIU Song,XING Rong-e,CAI Sheng-bao,YU Hua-hua,LI Peng-cheng
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| Abstract: |
| Abstract: The O·-2and ·OH scavenging activities of the complexes of sulfation chitosan with copper(Ⅱ) or zinc(Ⅱ) and DMW chitosan were investigated. Colorimetric method was used, where phenzine methosulphate (PMS)/dinucleotide reduced (NADH) and Fe/H2O2 were used as producing reagents and Nitro blue tetrazolium (NBT) and Fanhonghua as detecting reagents for O·-2 and ·OH. As expected, we obtained several satisfying results, as follow: Firstly, compared with DMW chitosan, the complexes of sulfation chitosan with copper(Ⅱ) or zinc(Ⅱ) had the stronger inhibition effect on O·-2. For example, when mass concentration of samples was 0.025 g/L, the O·-2 scavenging activity of the complexes of sulfation chitosan with copper(Ⅱ) and the complexes of sulfation chitosan with zinc(Ⅱ) were 94.18% and 93.19% respectively. Secondly, scavenging activity of the complexes of sulfation chitosan with copper(Ⅱ) or zinc(Ⅱ) (67.39%, 60.46%, 3.2 g/L) on ·OH was between low molecular weight chitosan (20 ku,88.06%, 3.2 g/L) and high molecular weight chitosan (761 ku, 18.71%, 3.2 g/L). Thirdly, low molecular weight chitosan had stronger scavenging effect on O·-2and ·OH than high molecular weight chitosan. At 1.6 g/L, the scavenging activity of low molecular weight chitosan (20 ku) on O·-2reached 54.69%, while that of high molecular weight chitosan (761 ku ) was 35.50%. Finally, the scavenging activity of several samples increased with increase in their mass concentration. However, the complexes of sulfation chitosan with copper(Ⅱ) or zinc(Ⅱ) had a quite strong scavenging action to O·-2, their scavenging activity barely had obvious changing over 0.025 g/L. This result also showed that the complexes of sulfation chitosan with metalion were very effective antioxidant.
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| Key words: chitosan the complexes of sulfation chitosan with metalion superoxide radical hydroxyl radical antioxidant activity |
