| 引用本文: | 王芳,董乐,戴聪杰,林娈,欧巧慧.桐花树多酚氧化酶同源基因片段的克隆及序列分析[J].海洋科学,2009,33(12):44-49. |
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| 桐花树多酚氧化酶同源基因片段的克隆及序列分析 |
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王芳1, 董乐1, 戴聪杰1, 林娈1, 欧巧慧1
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泉州师范学院
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| 摘要: |
| 以桐花树(Aegiceras corniculatum (L.) Blanco)叶为材料, 采用同源PCR克隆策略, 扩增桐花树多酚氧化酶(Polyphenol Oxidase, PPO)铜结合区之间的cDNA序列。该序列长度597 bp (Genbank accession No. GQ404509), 编码了一段由199 个氨基酸残基组成的多酚氧化酶保守区片段。桐花树PPO保守区片段具有PPO铜结合区A(CuA)的特征信号序列Hnswl.FFpFHRyyLyffE。同源比对表明, 该序列与GenBank上其他已有的植物多酚氧化酶氨基酸序列的同源性都在65%以上。
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| 关键词: 桐花树(Aegiceras corniculatum (L.) Blanco) 多酚氧化酶 基因克隆 序列分析 |
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| 基金项目:福建省高校服务海西建设重点项目 (A101);福建省科技厅青年科技人才创新项目(2006F3113);福建省自然科学基金资助项目(2008J04011);福建省教育厅科技计划项目(2007JA07151);泉州市科技局技术研究与开发项目(2007N6);泉州师范学院重点学科建设经费资助项目 |
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| Cloning and sequence analysis of polyphenol oxidase homologous gene from Aegiceras corniculatum (L.) Blanco |
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WANG Fang,DONG Le,DAI Cong-jie,LIN Luan,OU Qiao-hui
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| Abstract: |
| A PCR-based homologous cloning strategy was used to identify Polyphenol Oxidase (PPO) genes from the leavess of Corniculate Aegiceras (Aegiceras corniculatum (L.) Blanco ). The results of sequence analysis indicate that A 597 bp cDNA with a Cu-binding region encoding a 199 amino acids actin ortholog was successfully cloned and characterized (GenBank accession No. GQ 404511). The PPO contains the CuA-binding region signature sequence of PPO family (Hnswl.FFpFHRyyLyffE). Multiple alignment analysis of amino acid sequences of the putative PPO conserved regions in A. corniculatum was more than 65% homologous with the best scoring results from similarity searches of sequence databanks.
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| Key words: Aegiceras corniculatum polyphenol oxidase gene cloning sequence analysis |
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