| 摘要: |
| 用光生物反应器与光照培养箱两种培养容器培养雨生红球藻(Haematococcus pluvialis), 对雨生红球藻在两种容器培养过程中细胞生长、pH 值、溶解氧(DO)及虾青素积累情况进行比较, 同时比较不同接种密度对光生物反应器培养效果的影响。实验表明, 光生物反应器中藻细胞的调整期较短, 接种1 d 后即进入指数生长阶段, 在胁迫阶段则仅需4 d 即达到虾青素含量的峰值; 将pH 值控制在偏碱性条件下(7.75±0.10)有利于藻细胞更好生长; 营养培养阶段DO相对饱和度上升至80%, 而在胁迫阶段则迅速降低, 最低值小于6%; 较高的接种密度(2.3×104 个/mL)具有较短的营养培养周期(7 d), 且因接种密度变化对胁迫周期长短无明显影响(均为4 d), 选用较高的接种密度可望降低工业生产成本。 |
| 关键词: 雨生红球藻(Haematococcus pluvialis) 虾青素 光生物反应器 培养 积累 |
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| 基金项目:厦门市科技项目(3052Z20031086); 新加坡厦门大学校友基金会项目(2007); 2007 年度国家大学生创新性实验计划项目(2007);第十一届“挑战杯”全国大学生课外学术科技作品竞赛(2009); 厦门大学海洋学系“海洋”本科生科研奖励计划(2008) |
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| Haematococcus pluvialis culture in photobioreactor |
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SHEN Yuan,CAI Ming-gang,HUANG Shui-ying,SHI Rong-gui,LI Zhe,LU Xiao-xia
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| Abstract: |
| The green microalga Haematococcus pluvialis was cultured in photobioreactor and light incubator, respectively, to compare the effect on cell growth and astaxanthin accumulation. The effects of pH, dissolved oxygen (DO), and effects of different inoculating densities were also studied. The results indicated that only one day was needed for algae in photobioreactor to enter exponential growth phase and only four days were required to reach the maximum of astaxanthin content during stress stage. The culture period of algae in photobioreactor was shorter than that in light incubator. Slight alkaline condition (pH =7.75 ±0.10) was beneficial to the cell growth. DO increased up to 80% during nutrient culture stage and decreased rapidly during stress stage with a minimum of less than 6%. Since the higher cell inoculum density (2.3×104 cell/mL) showed shorter nutrient culture period (7 days) and different cell inoculum densities had no effect on the length of stress period (only 4 days were needed), we suggested that higher cell inoculum density might be adopted for reducing costs and shorten cycles of algae production. |
| Key words: Haematococcus pluvialis astaxanthin photobioreactor culture accumulation |
