| 摘要: |
| 对从南海海藻内生真菌ZJ27 次级代谢产物中分离得到的5 种化合物进行抑菌活性的研究, 采用96 孔板法测定化合物对蜡状芽孢杆菌、枯草芽孢杆菌、大肠杆菌、白色葡萄球菌、藤黄八叠球菌、金黄色葡萄球菌等6 种细菌的抑制活性, 并用生长速率法测定化合物对黑曲霉、尖孢镰刀菌、立枯丝核菌和白色念珠菌等4 种真菌的抑制活性。化合物对上述6 种细菌均有不同的抑制作用, 其中化合物1对枯草芽孢杆菌、白色葡萄球菌和藤黄八叠球菌有很好的抑制作用, MIC 值分别为0.1、0.2、0.2μmol/mL。化合物1 和2 对黑曲霉、尖孢镰刀菌、立枯丝核菌和白色念珠菌也有很好的抑制作用, 化合物1 对这4 种真菌的IC50分别为15.06、3.09、3.68、8.19 μmol/mL, 化合物2 相应的IC50 为22.28、21.32、8.18、21.75 μmol/mL。化合物1 和2 作为天然抗菌物质, 具有广谱抑菌效果, 具有较高的应用潜力和综合开发前景。 |
| 关键词: 海洋真菌 次级代谢产物 抑菌活性 |
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| 基金项目:国家自然科学基金项目(20972197), 广东省自然科学基金项目(9151027501000055), 广东省科技计划项目(2010B030600004),广东海洋大学基金项目(E09095) |
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| Antibacterial activity of secondary metabolites of marine fungi ZJ27 |
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| Abstract: |
| The antibacterial activities of five compounds isolated from secondary metabolites of seaweed endophytic fungi ZJ27 were studied in Bacillus cereu, Bacillus subtilis, Escherichia coli, Staphylococcus albus, Sarcina luteus, and Staphylococcus aureus and the antifungal activities were tested in Aspergillus niger, Fusarium oxysporum, Rhizoctonia solani, and Candida albicans by the growth rate method. Different compounds had diffident antibacterial activities in the six bacteria. Compound 1 showed remarkable inhibition of B. subtilis, S. albus and S. luteus with an MIC of 0.1, 0.2, and 0.2 μmol/mL, respectively. Compounds 1 and 2 had notable inhibition of A. niger, F. oxysporum, R. solani and C. albicans, with the IC50 values of compound 1 being 5.06, 3.09, 3.68, and 8.19 μmol/mL and those of compound 2 were 22.28, 21.32, 8.18, and 21.75 μmol/mL. As natural antibacterial substances, Compounds 1 and 2 have a great range of potential utilities and a prospect of development and are worth further study. |
| Key words: marine fungi secondary metabolites antibacterial activity |
