| 引用本文: | 胡 静,齐兴柱,尹绍武,骆 剑,朱晓平,祝 斐,胡亚丽.波纹唇鱼mtDNA D-loop 序列变异分析[J].海洋科学,2012,36(4):50-56. |
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| 摘要: |
| 为研究波纹唇鱼(Cheilinus undulatus)线粒体D-loop 序列遗传多样性, 作者采用聚合酶链式反应和克隆技术对海波纹唇鱼群体(n=25)的mtDNA D-loop 序列进行克隆和测序, 获得长度大约为1 400 bp的产物。用ClustalX 软件进行排序比较, 将结果导入MEGA5.0, 结果显示出在这25 尾个体中, 共检测出66 个变异位点, 包括0 个碱基缺失, 4 个碱基插入, 59 转换位点, 2 颠换位点及1 个转换和颠换同时存在的位点。并用MEGA5.0 软件构建该群25 尾个体的NJ 和UPGMA 系统树。由DNASP 软件计算出该波纹唇鱼群体的多态位点数(S)为62, 核苷酸多样性(Pi)为0.00606, 平均核苷酸差异数为6.907。结果表明波纹唇鱼群体的mtDNA-loop 基因个体差异程度不明显。 |
| 关键词: 波纹唇鱼(Cheilinus undulatus) mtDNA D-loop 遗传多样性 |
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| 基金项目:国家自然科学基金项目(40966003); 海南省自然科学基金(809009); 海南省热带水生生物技术重点实验室开放基金(shkyjj0801); 海南大学211工程研究生创新平台课题资助 |
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| The variation analysis of mtDNA D-loop sequence of Cheilinus undulatus |
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| Abstract: |
| The aim of this paper was to study the variations in the mtDNA D-loop sequences of Cheilinus undulatus populations. Twenty-five individuals of C. undulatus were collected from the offshore area of Hainan province. One thousand and four hundreds bp nucleotide sequences of mtDNA D-loop region were obtained using PCR and cloning techniques. 66 variation sites including 0 deletion, 4 inserts, 59 transition sites, 2 transversion sites and 1 transition-transversion sites were observed using ClustalX and MEGA5.0 softwares. The pairwise genetic distances of these 25 individuals were calculated using the MEGA5.0 software and based on that, the NJ phylogenetic trees was contracted for these fish populations. Analysis of this fish populations using the DNASP software showed that they had a polymorphic sites (S) of 62, a nucleotide diversity (Pi) of 0.00606 and an average number of nucleotide differences (K) of 6.907, respectively. Overall, there were no significant variations between mtDNA D-loops of C. undulatus populations. |
| Key words: Cheilinus undulatus mitochondrial DNA D-loop genetic diversity |
