| 引用本文: | 苏 艳,段继周,段东霞,蔺存国,张 杰,侯保荣,杨东方.材料表面性质对微生物附着行为的影响[J].海洋科学,2012,36(12):56-63. |
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| 摘要: |
| 利用荧光显微镜计数法, 研究了微生物在4 种不同材料表面的初始附着行为, 对微生物附着进行了定量描述, 初步获得以下结果: (1)不同材料表面, 细菌的附着量呈现如下差异: 316L 不锈钢>PVC>玻璃, 在有机硅材料(道康宁T2)表面的附着变动性很大; (2)同种材料表面, 不同细菌的附着量也不同, 表现为: Shewanella oneidensis MR-1 菌株和Pseudomonas aeruginosa PAO1 菌株均大于Escherichia coli JM109 菌株。并由此对其附着差异和影响因素进行了讨论。 |
| 关键词: 微生物附着 荧光显微镜计数 有机硅 不锈钢 玻璃 聚氯乙烯 |
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| 基金项目:中国科学院重要方向性项目(No. KZCX2-EW-205); 国家自然科学基金项目(40976046) |
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| Influence of different material surfaces on microbial adhesion |
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| Abstract: |
| Biofouling has great impact on marine vessels and engineering facilities, resulting in huge losses to the development of marine economy. It is very important to study the regular pattern of biofouling and develop new antifouling materials for controlling biofouling. Here we studied the initial attachment behavior of bacteria on 4 different materials surface with fluorescence microscopy. The results show that: (1) The attachment ability of bacteria on different kinds of materials is different with an order: 316L stainless steel > PVC > Glass. It is worth mentioning that the bacteria attachment ability on silicone material (Dow Corning T2) varied widely. (2) Different kinds of bacteria also show different attachment ability on the same material surface. It is easier for S. Oneidensis MR-1 and P. aeruginosa PAO1 strain to adhere on materials than E. lico JM09 strain. In this paper, the different attachment ability of bacteria and the influence factors were discussed. |
| Key words: Microbial adhesion Count by fluorescence microscope Silicone Stainless steel Glass PVC |
