引用本文:	史松富,姚韩韩,林志华,周小龙,齐晓艳,董迎辉.24个泥蚶EST-SSR标记的开发与分析[J].海洋科学,2013,37(8):42-46.

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24个泥蚶EST-SSR标记的开发与分析
史松富^1,2, 姚韩韩¹, 林志华¹, 周小龙^1,2, 齐晓艳³, 董迎辉¹
1.浙江万里学院浙江省水产种质资源高效利用技术研究重点实验;2.上海海洋大学水产与生命学院;3.宁波大学海洋学院

摘要:

利用3 000条泥蚶(Tegillarca granosa)单拷贝序列, 筛选到132条含有SSR 候选位点序列, 成功设计引物50条。群体多态性检测结果显示, 24个SSR位点为多态性, 各位点的等位基因介于2～5个, 平均等位基因数3.5个; 观测杂合度Ho、期望杂合度He、多态信息含量PIC范围分别为0.000～0.591、0.045～0.727、0.043～0.696; 多态位点中有11个高度多态、4个中度多态、9个低度多态; 进行哈迪-温伯格平衡检测后, 用Bonferroni correction法进行校正, 结果有17个位点显著偏离平衡。

关键词: 泥蚶(Tegillarca granosa) EST-SSR 单拷贝序列遗传多样性

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基金项目:国家现代贝类产业技术体系项目(CARS-48); 浙江省农业新品种选育重大科技专项(2012C12907-4); 宁波市科技创新团队项目(2011B82017); 宁波市科技局农业择优委托项目(2010C10011)

Characterization and analysis of 24 polymorphic EST-SSR loci in Tegillarca granosa

Abstract:

50 pairs of primers were designed successfully by using 132 candidates of SSR loci sequences which were identified from 3000 single copy sequences of Tegillarca granosa. It was revealed from the investigation of polymorphism that 24 SSR loci were polymorphic. The number of alleles of the 24 EST-SSR loci varied from 2 to 6, and the mean value is about 3.5. The range of the observed heterozygosity, the expected heterozygosity and the polymorphic information content was 0.000 to 0.591, 0.045 to 0.727 and 0.043 to 0.696, respectively. There are 9 high polymorphic SSR locus, 4 moderate polymorphic SSR locus, and 11 high polymorphism SSR locus. 17 SSR locus are significant deviations from Hardy-Weinberg equilibrium after Bonferroni correction.

Key words: Tegillarca granosa EST-SSR loci single copy sequence polymorphism