| 摘要: |
| 通过研究不同体质量仿刺参(Apostichopus japonicus)的免疫活性, 为仿刺参生长中免疫指标的建立提供依据。实验选取了50、100、150 g 左右的仿刺参各10 只, 测定其体腔细胞数量、各细胞类群比例及部分免疫酶活性。结果表明: 不同体质量的仿刺参, 其体腔细胞数量及各细胞类群、过氧化物酶(POD)、超氧化物歧化酶(SOD)具有显著性变化, 而体腔液中酚氧化酶(PO)活性极低。体质量150 g左右仿刺参的细胞数量和SOD活性较其他两组都明显升高, 细胞类群也有明显变化(P<0.017), 但POD活性显著降低(P<0.05)。不同体质量的仿刺参免疫活性具有显著性差别, 说明了仿刺参免疫组分在生长过程中不断变化, 免疫防御能力在各阶段有所差别。 |
| 关键词: 仿刺参 体质量 免疫活性 |
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| Study on immune activity of sea cucumber (Apostichopus japonicus) with different weight |
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| Abstract: |
| In order to provide basis knowledge on immune system of sea cucumber (Apostichopus japonicus), the immune activity in sea cucumber with different body weight were studied. Three groups of sea cucumbers (50 g, 100 g, and 150 g) were selected in the experiment (10 individuals each). The total coelomocytes numbers, the ratio of various types of coelomocytes and some immune enzyme activities were determined. The results showed that the body weight had a significant effect on total coelomocytes amount, the ratio of various types of coelomocytes, POD and SOD activity, while the PO activity in coelomic fluid of sea cucumber was extremely low. The total coelomocytes amount and SOD activity in 150 g group of sea cucumber were significantly higher than the other two groups, but the POD activity was significantly lower than the other two groups (P<0.05). The ratio of various types of coelomocytes in 150 g group had significant difference compared to other groups (P<0.017). In a word, the body weight had significant impacts on immune activity of sea cucumber. The immune components of sea cucumber changed in the process of development and the immune defense ability might vary in different stages. |
| Key words: Apostichopus japonicus body weight immune activity |
