| 摘要: |
| 研究7 种海黍子(Sargassum kjellmanianum)乙酸乙酯相提取物的抗菌活性和对小鼠免疫细胞功能的影响, 筛选出抗菌和体外免疫活性较强的提取物。考察7 种提取物的抗菌活性, 以及对小鼠腹腔巨噬细胞RAW264.7 增殖、脾淋巴细胞增殖、释放NO、吞噬中性红能力的影响。抑菌实验结果表明, 7 种提取物对真菌无明显的抑制作用, 而针对不同的细菌表现出不同的程度的抑制活性; 免疫实验结果表明, 乙酸乙酯相的7 个样品都具有一定的体外免疫活性, 不但可以促进小鼠巨噬细胞RAW264.7 增殖和体外刺激淋巴细胞增殖, 还可以促进小鼠腹腔巨噬细胞产生NO和吞噬中性红。 |
| 关键词: 海黍子(Sargassum kjellmanianum) 硅胶柱层析 抗菌活性 体外免疫活性 |
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| 基金项目:山东省优秀中青年科学家科研奖励基金2008BS06012; 山东省自然科学基金(ZR2009BL030); 烟台市科技发展计划项目(2008GGA0020080410084458) |
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| Antimicrobial and in vitro immunomodulating activity of extracts from ethyl acetate phase of Sargassum kjellmanianum |
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| Abstract: |
| In this paper, the antimicrobial and immunomodulating activities of the extracts from ethyl acetate phase of Sargassum kjellmanianum were studied to screen out the extracts with strongest activities. The antimicrobial activity of these seven extracts as well as their effects on proliferation of mice abdominal macrophage RAW264.7 and spleen lymphocytes, release of nitro oxide (NO), ability to devour neutral red were investigated. The results showed that these seven extracts had little inhibitory effect on the fungus, while showed different inhibitory effect towards different bacterium. All of these 7 extracts showed immunomodulating activity. They could stimulate the prolifertion of both mice macrophage RAW264.7 and the spleen lymphocytes. They could stimulate the mice abdominal macrophage to produce NO and devour the neutral red. |
| Key words: Sargassum kjellmanianum silicagel column chromatography antimicrobial activity in vitro immunomodulating |
