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| 引用本文: | 陶翠花,刘莹莹,赵丽媛,许 敏,祝 茜.绿海龟α-actin 基因的cDNA 克隆与序列分析[J].海洋科学,2014,38(3):98-103. |
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| 绿海龟α-actin 基因的cDNA 克隆与序列分析 |
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陶翠花1, 刘莹莹2, 赵丽媛1, 许 敏1,2, 祝 茜1,2
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1.国家海洋局 第三海洋研究所;2.山东大学(威海) 海洋学院
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| 摘要: |
| 为探究绿海龟(Chelonia mydas)α-actin 基因序列的相关信息, 作者利用RT-PCR 和RACE 方法从绿海龟肌肉组织中获得了α-actin 基因的cDNA 全长序列, 共1347bp(GenBank 登录号为JX073650)。所得序列包含一个1134 bp 的开放阅读框, 编码由377 个氨基酸组成的蛋白, 该蛋白7~377 位为Actin 结构域, 14~17 位有一个糖基化位点, 无信号肽; 预测分子量为42.0 kDa, 理论等电点为5.23。将编码区序列与GenBank 上同源序列进行比对发现, 核苷酸序列相似性均在85.4%以上, 氨基酸序列相似性均在98.9%以上, 说明α-actin 基因作为编码蛋白是高度保守的。 |
| 关键词: 绿海龟(Chelonia mydas) α-actin 基因 RACE 技术 |
| DOI：10.11759/hykx20121006001 |
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| 基金项目:国家海洋局第三海洋研究所基本科研业务费专项资金项目(2010006); 国家海洋公益性行业科研专项(201105011) |
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| Cloning and sequence analysis of full-length cDNA of α-actin gene from Chelonia mydas |
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| Abstract: |
| To explore the sequence and characteristic of α-actin gene from Chelonia mydas, the full-length cDNA sequence of α-actin gene was cloned using RT-PCR and RACE technique, which was consisted of 1347 bp nucleotides (GenBank accession number: JX073650), with a putative open reading frame (ORF) of 1134 bp encoding a deduced 377 amino acid protein containing a glycosylation site (from 14 to 17) and an Actin domain (from 7 to 377). The molecular weight of the protein was 42.0 kDa and the isoelectric point (pI) was 5.23. The nucleotide sequence similarity of α-actin gene between C. mydas and other species was above 85.4%, while the similarity of amino acid sequence was more than 98.9%, suggesting that α-actin gene was highly conserved. This study has enriched the Actin gene database and provided basic data for further studies on expression and function of relevant genes. |
| Key words: Chelonia mydas α-actin gene RACE |
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