| 摘要: |
| 在室内控温条件下测定了定向选育获得的刺参(Apostichopus japonicus)耐高温群体子一代高温胁迫下的存活率及热休克蛋白(Hsp90 和Hsp26)基因表达特征, 从生理及分子水平分析了刺参耐高温群体子一代对高温胁迫的响应特征, 确定高温耐受性的可遗传性。结果表明: 与野生刺参群体子一代相比, 定向选育刺参群体子一代在高温胁迫下表现出较高的存活率, 32℃高温刺激下定向选育群体和野生群体稚参的存活率分别为80%和56.67%。高温胁迫下两个群体子一代的热休克蛋白90 及26 基因表达特征也存在差异, 定向选育刺参群体子一代在高温刺激条件下热休克蛋白基因表达水平低于野生群体, 且表达出现滞后现象。结果表明定向选育刺参群体子一代对高温具有一定的耐受能力, 一定程度上验证了高温耐受性的可遗传性, 该实验所获得的结果可以为后续刺参良种培育提供一定的理论依据和技术支撑。 |
| 关键词: 刺参(Apostichopus japonicus) 定向选育 存活率 热休克蛋白 耐温性 |
| DOI：10.11759/hykx20121105001 |
| 分类号: |
| 基金项目:国家“863”计划项目(2012AA01A412); 农业科技成果转化资金项目(2012GB24910656); 山东省农业良种课题速生、抗病、耐高温刺参良种选育; 国家自然科学基金项目(40976089) |
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| The study on thermo tolerance of juvenile offspring Apostichopus japonicus (Selenka) with directive breeding |
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| Abstract: |
| The survival rate of the juvenile offspring Apostichopus japonicus in directive breeding population and the Hsp90 and Hsp26 gene expression were determined and compared under the condition of indoor temperature control, which were used to detect the heritability of thermo-tolerance in directive breeding population. It was found that the offspring in directive breeding population exhibited high survival rate compared to wild population with exposure to high temperature. The survival rate of offspring in directive breeding population and wild population was 80% and 56.67%, respectively when exposured to 32℃. The transcripts of Hsp90 and Hsp26 in these two populations were also different. Throughout the experiment, the Hsp90 and Hsp26 gene expressions in directive breeding population were lower than those in wild population, and in directive breeding population the maximum transcripts of Hsp90 and Hsp26 appeared at 2 h, however the peak of Hsp90 and Hsp26 gene expression reached at 1 h in wild population. It is indicated that the juvenile offspring of directive breeding population also possess the ability of thermo-tolerance. The results of this study will provide information for further research on selective breeding of sea cucumber. |
| Key words: Apostichopus japonicus directive breeding survival rate heat shock proteins thermo-tolerance |
