| 摘要: |
| dpp是转化生长因子β 家族的重要成员, 在胚胎发育中作为形态发生子参与体轴形成及附肢发育等过程。为了研究牡蛎dpp同源基因在贝壳发生中的作用, 揭示贝壳发生的分子机理, 作者克隆了一种长牡蛎(Crassostrea gigas)的dpp基因, 命名为cgdpp。序列和进化分析显示, cgdpp分子中不同位点的氨基酸残基变异程度不同。作者用整装原位杂交的手段研究了dpp同源基因在长牡蛎早期发育中的表达情况。结果表明, dpp同源基因参与了从贝壳形成区开始分化到早期贝壳形成的全过程。在担轮幼虫(Trochophore)中, dpp同源基因似乎调控了贝壳的形状与扩张速度; 在早期D形幼虫中, dpp同源基因表达量突然下降至痕量水平, 并与贝壳发育区无明显的关联, 提示dpp同源基因可能仅仅参与了贝壳的发生, 不参与其进一步的发育过程。 |
| 关键词: 长牡蛎(Crassostrea gigas) 发育 整装原位杂交 转化生长因子 |
| DOI：10.11759/hykx20130205002 |
| 分类号: |
| 基金项目:国家自然科学基金项目(31001102); 国家973 计划项目(2010CB126403) |
|
| Clone of dpp gene and its functions in larval shell formation of the Pacific oyster Crassostrea gigas |
|
|
| Abstract: |
| Dpp is an important member of the transforming growth factor (TGF) β family, which acts as a morphogen in many animals. It was revealed that dpp gene was involved in the shell formation of mollusks. The Pacific oyster (Crassostrea gigas) is a world-wide economic bivalve species, and is a potential model organism of bivalves. In this work, we cloned a dpp-homolog from C. gigas (cgdpp), which exhibited typical characteristics of TGF-β members. The expression pattern of cgdpp was investigated in the early development of C. gigas using whole mount in situ hybridization. The results indicated that cgdpp might participate in cell motility and differentiation during development of the shell field. In trochophore, the cgdpp might regulate the shape and extension of the shell. Only trace mount of cgdpp was detected in the early D-veliger, suggesting that the cgdpp is not involved in the later development of shells since D-veliger. In addition, different from previous report, our results did not support the involvement of cgdpp in the formation of the shell hinge. Our studies demonstrated the roles of cgdpp in the shell formation of C. gigas, which would help to reveal the mechanism of shell formation. |
| Key words: Crassostrea gigas development whole mount in situ hybridization transforming growth factor |
