| 摘要: |
| 为研究水温对刺参(Apostichopus japonicus)消化酶及免疫酶活力的影响, 本实验将体质量为8.0 g左右的刺参分别于10、14、18、22℃下养殖4 周后, 对其消化道中蛋白酶、淀粉酶、纤维素酶和脂肪酶活力, 以及体腔液中溶菌酶和超氧化物歧化酶活力进行测定。研究结果显示, 刺参消化道中蛋白酶含量最高, 其酶活在10℃下最高, 但随着温度的升高活力逐渐下降, 在水温升至18℃以后酶活显著下降; 淀粉酶活力在水温升至的18℃时无明显变化, 但至22℃时活力显著下降; 纤维素酶和脂肪酶活力较小, 不同温度下其活力无显著差异。水温对刺参体腔液溶菌酶活性影响显著, 在水温为18℃时达到最高值, 升至22℃时其活力显著降低; 超氧化物歧化酶活性在水温为18℃达到最低, 但水温升至22℃其酶活与18℃相比又显著升高。 |
| 关键词: 水温 刺参(Apostichopus japonicus) 消化酶 免疫酶 |
| DOI：10.11759/hykx20140310003 |
| 分类号: |
| 基金项目:山东省“两区”建设专项；山东省现代农业产业技术体系虾蟹类创新团队项目(SDAIT-15-011-06) |
|
| Effects of water temperature on activities of digestive enzymes and immune enzymes in Apostichopus japonicus |
|
|
| Abstract: |
| To investigate the effects of temperatures on the digestive enzymes and immune enzymes activities, several groups of sea cucumbers (Apostichopus japonicus) (approximately 8.0 g) were reared under different constant temperatures ranged from 10～22℃ for four weeks. Then, the activities of protease, amylase, cellulase and lipase in the digestive tract and the lysozyme and superoxide dismutase (SOD) in coelomic fluid were determined. The results showed that the protease was the most abundant enzyme in the digestive tract. The protease activity was highest when the sea cucumbers were reared in 10℃, but it was significantly inhibited when the temperature was raised to 18℃ (P<0.05). There was no significant difference in amylase activity between sea cucumbers groups reared in temperature below 18℃. However, the amylase activity was significant reduced in the sea cucumber group when temperature was increased to 22℃ (P<0.05). The levels of cellulose and lipase activities were extremely low in all experimental groups. Moreover, it was found that the lysozyme activity was the highest when the sea cucumbers were reared in 18℃ (P<0.05), but it was significantly decreased at 22℃(P<0.05). However, activity of SOD exhibited a different trend in sea cucumber groups. The SOD activity was gradually reduced as the temperature rose, but it was significantly increased when temperature rose up to 22℃ (P<0.05). |
| Key words: water temperature Apostichopus japonicus digestive enzyme immune enzyme |
