首页 | 期刊介绍 | 编委会 | 道德声明 | 投稿指南 | 常用下载 | 过刊浏览 | In English
引用本文:王洪斌,李信书,宋秀梅,吴 言,李士虎,阎斌伦.海州湾条斑紫菜养殖对浮游藻类群落结构及遗传多样性的影响[J].海洋科学,2014,38(12):103-109.
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 1713次   下载 2095 本文二维码信息
码上扫一扫!
分享到: 微信 更多
海州湾条斑紫菜养殖对浮游藻类群落结构及遗传多样性的影响
王洪斌1,2, 李信书1, 宋秀梅1, 吴 言1, 李士虎1, 阎斌伦2
1.淮海工学院 海洋学院;2.江苏省海洋生物技术重点实验室
摘要:
为了研究条斑紫菜(Porphyra yezoensis)养殖对江苏海州湾浮游藻类群落结构及遗传多样性的影响, 于江苏省海州湾条斑紫菜养殖及非养殖海区各设置4 个采样位点, 采集表层海水, 提取海水中浮游藻类的总DNA, PCR 扩增浮游藻类核酮糖1, 5-二磷酸羧化/氧化酶大亚基(rbcL) 基因片段, 构建了养殖及非养殖海区rbcL片段质粒文库。在文库中随机选择50 个阳性克隆并测序。经比对分析, 在条斑紫菜非养殖区发现8 种海洋微藻, 隐鞭藻占总克隆数22%, 海链藻和骨条藻分别占6%和2%; 养殖海区发现10 种微藻, 其中异丝藻占总克隆数22%, 优势度明显, 仅3 种微藻为二海区共有(骨条藻、隐鞭藻及小球藻), 表明紫菜养殖及非养殖区浮游藻类群落组成差异显著。条斑紫菜非养殖区及养殖区的浮游藻类香农指数均值分别为3.273 和3.654, 均匀度指数均值分别为1.090 和1.040, 显示养殖区浮游藻类遗传多样性较丰富, 而群落的成熟度与稳定性非养殖区高。研究证实, 表层海水浮游藻类群落结构及组成是动态的, 条斑紫菜的养殖行为形成浮游藻类群落结构及遗传多样性较大差异性。
关键词:  条斑紫菜(Porphyra yezoensis)  浮游藻类  核酮糖1, 5-二磷酸羧化/氧化酶大亚基基因  遗传多样性
DOI:10.11759/hykx20130922001
分类号:
基金项目:江苏省科技支撑-社会发展项目(BE2011719); 江苏高校优势学科建设工程资助项目; 国家级高等学校大学生创新创业训练计划项目(201311641002z)
Effect of Porphyra yezoensis farming on the structure and genetic diversity of phytoplankton community in Haizhou Bay
Abstract:
In order to investigate the effect of Porphyra yezoensis farming on the structure and genetic diversity of phytoplankton community in Haizhou Bay in Jiangsu, four sampling sites were set in each P. yezoensis breeding and non-breeding sea in Haizhou Bay in Jiangsu Province in December 2012. The surface water was collected and the total DNA of seawater was extracted. The planktonic algae ribulose 1, 5-bisphosphate carboxylase/oxide large subunit (rbcL) gene was amplified using PCR, which was used to construct a plasmid library of breeding and non-breeding sea rbcL fragment. 50 positive clones of the library were selected randomly and sequenced. After a comparative analysis, eight species of marine microalgae were found in non-breeding areas, among which, Cryptomonas made up 22% of the total number of clones, Thalassiosira and Skeletonema were smaller proportion, accounting for 6% and 2%, respectively. 10 kinds of marine microalgae were found in breeding sea, among which Heterosigma has the obvious superiority, accounting for 22% of the total number of clones. Only three kinds of microalgae (Skeletonema, Cryptomonas and Chlorella) were found in both breeding areas and non-breeding areas, suggesting that phytoplankton community composition was significantly different between breeding areas and non-breeding areas. The average Shannon index of sea phytoplankton in P. yezoensis non-breeding and breeding areas was 3.273 and 3.654, respectively. The average evenness index was 1.090 and 1.040, respectively, suggesting breeding areas were rich in phytoplankton with genetic diversity, while the maturity and stability of the community were high in non-breeding areas. Our study confirmed that the structure and composition of phytoplankton community is dynamic, and P. yezoensis farming changed community structure and genetic diversity of phytoplankton.
Key words:  Porphyra yezoensis  Phytoplankton  Ribulose 1, 5-bisphosphate carboxylase/ xidase large subunit gene  Genetic diversity
版权所有 《海洋科学》 Copyright©2008 All Rights Reserved
主管单位:中国科学院 主办单位:中国科学院海洋研究所
地址:青岛市市南区福山路32号  邮编:266071  电话:0532-82898755  E-mail:marinesciences@qdio.ac.cn
技术支持:北京勤云科技发展有限公司