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| 氮磷硅对咖啡双眉藻和缢缩菱形藻繁殖速度和油脂积累的影响 |
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王瑶华1, 吴洪喜2,3, 黄振华2,3, 陈肖肖2,3, 胡 园2,3, 王召根1,3
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1.上海海洋大学 水产与生命学院;2.浙江省海洋水产养殖研究所;3.浙江省近岸水域生物资源开发与保护重点实验室
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| 摘要: |
| 采用光密度法和尼罗红染色法, 探索了氮、磷、硅对从温州海区分离出的底栖硅藻咖啡双眉藻(Amphora coffeaeformis)和缢缩菱形藻(Nitzschia constricta)的繁殖速度和油脂积累的影响。结果显示, 2种底栖藻在NO3-N 质量浓度为1.746 mg/L 时, 细胞均不繁殖, 且在实验第7 天开始死亡, 当 NO3-N 质量浓度再升高后, 细胞繁殖速度开始加快, 质量浓度达8.820 mg/L 后, 藻细胞的繁殖速度不再明显加快; 2 种藻类的繁殖速度均随H2PO4-P 质量浓度升高而加快, 但当质量浓度达到0.884 mg/L 后, 藻细胞的繁殖速度不再明显加快; 2 种底栖藻类均随SiO3-Si 质量浓度增加而加快, 但质量浓度对2 种底栖硅藻细胞繁殖速度影响并不显著。3 种元素对2 种底栖硅藻繁殖速度的影响力: N>P>Si。实验期间,2 种底栖硅藻的油脂含量均呈先降后升的变化规律, 油脂的积累主要集中在实验后期, 氮磷硅限制均能促进2 种底栖硅藻细胞油脂的积累, 对2 种硅藻细胞油脂积累的影响程度, 氮限制比磷和硅限制都要大。 |
| 关键词: 氮磷硅 尼罗红染色 繁殖 油脂 |
| DOI：10.11759/hykx20140224002 |
| 分类号: |
| 基金项目:浙江省近岸水域生物资源开发与保护重点实验室人才培养基金项目(J2012010) |
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| Influence of Nitrogen, Phosphorus and Silicon on reproducing rate and lipid accumulation of Amphora coffeaeformis and Nitzschia constricta |
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| Abstract: |
| The effects of Nitrogen, Phosphorus and Silicon on reproduction rate and lipid accumulation of Amphora coffeaeformis and Nitzschia constricta isolated from Wenzhou ocean using optical density method and Nile red fluorescence method were investigated. The results showed that the growth of these two kinds of benthic diatoms were inhibited at nitrogen concentration of 1.746 mg/L, and even died on the seventh day. However, the reproduction rate of these two kinds of benthic diatoms increased when the nitrogen concentration increased, however, the reproduction rate wasn’t significantly accelerated after the nitrogen concentration reached 8.820 mg/L. The reproduction rate of these two kinds of benthic diatoms was accelerated with increasing phosphorus concentrations, however, the reproducing rate was restricted when the nitrogen concentration reached 0.884 mg/L. The reproduction rate of two benthic diatoms was notinfluencedas increasing the silicon concentration. The order of the effect of three knids of nutritional elements was N>P>Si. Results also showed that the lipid contents of two kinds of benthic diatoms decreaseed first, and then increased, and the lipid accumulation is mainly concentrated at the late growth stage. The lipid accumulation capacity of these two kinds of benthic diatoms could be enhanced by nitrogen, phosphorus and silicon concentration limitation. The nitrogen limitation has a more significant effect than phosphorus and silicon limitation. |
| Key words: Nitrogen, Phosphorus and Silicon Nile red fluorescence reproduction lipid |