| 引用本文: | 凌晶宇,梁洲瑞,王飞久,孙修涛,汪文俊,刘福利,姚海芹.温度胁迫对掌状海带幼苗生长、抗氧化系统及叶绿素荧光的影响[J].海洋科学,2015,39(12):39-45. |
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| 温度胁迫对掌状海带幼苗生长、抗氧化系统及叶绿素荧光的影响 |
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凌晶宇1,2, 梁洲瑞2, 王飞久2, 孙修涛2, 汪文俊2, 刘福利2, 姚海芹1,2
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1.上海海洋大学水产与生命学院;2.中国水产科学研究院黄海水产研究所, 农业部海洋渔业可持续发展重点实验室
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| 摘要: |
| 采用掌状海带(Laminaria digitata)幼苗为实验材料, 研究了不同温度(3、8、13、18℃)对其生长、抗氧化系统及叶绿素荧光参数的影响。测定主要参数有: 相对生长速率(RGR)、可溶性蛋白、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、PSII 最大荧光产量(Fv/Fm)、最大相对电子传递速率(rETRmax)结果表明: (1)掌状海带幼苗的RGR 在13℃下达最大值, 与3℃处理组对比呈显著性差异(P<0.05)。(2)3℃处理4 h 或18℃处理36 h 对掌状海带幼苗SOD、CAT 活性有显著影响。(3)3℃培养条件下, 掌状海带幼苗的Fv/Fm、rETRmax值显著低于对照组(13℃); 18℃处理组的Fv/Fm值随着胁迫时间的延长而降低, 其rETRmax值在胁迫4 h 后达最大; 处理24 h, 8℃处理组的rETRmax值显著高于其他处理组。 |
| 关键词: 掌状海带(Laminaria digitata) 温度 生长速率 抗氧化系统活性 叶绿素荧光 |
| DOI：10.11759/hykx20150107001 |
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| 基金项目:国家863 计划高技术研究发展计划项目(2012AA10A406); 山东省科技项目(2013GGF01028); 国家自然科学基金青年科学基金项目(41306176) |
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| Effects of temperature stress on the growth, antioxidant system, and chlorophyll fluorescence of Laminaria digitata |
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| Abstract: |
| The aim of this study was to observe temperature stress effects in increments of 3℃, 8℃, 13℃, and 18℃ on the growth, antioxidant system, and chlorophyll fluorescence parameters of Laminaria digitata. The monitored variables included the relative growth rate (RGR), soluble protein content, maleic dialdehyde content, basal activities of antioxidant enzymes, optimal chlorophyll fluorescence quantum yield of Photosystem II (Fv/Fm), and the maximum relative electron transport rate (rETRmax). The results can be summarized as follows: (1) The highest RGR values were observed at 13℃ which was significantly different to the RGR values at 3℃. (2) Antioxidant enzyme activities were significantly different between treatments at 3℃ for 4h and at 18℃ for 36h. (3) The Fv/Fm andrETRmax values at 3℃ were significant lower than at 13℃. The Fv/Fm values decreased with stressed time and the highest rETRmax values were reached after 4h at 18℃. The rETRmax values at 8℃ were significant higher than the other groups after 36h of treatment. |
| Key words: Laminaria digitata temperature growth rate antioxidant system chlorophyll fluorescence |