| 摘要: |
| 为确立一种准确测量仿刺参体长的方法, 选用薄荷醇(menthol)为麻醉剂, 在不同麻醉浓度条件下, 观察了对仿刺参(Apostichopus japonicus)幼参的麻醉反应, 同时比较了在不同水温(11、13、15、17、19、21℃)环境条件下对不同规格((L) 42.37 g±1.99 g、(M) 22.91 g±1.03 g、(S) 12.09 g±1.51g)幼参的麻醉效果。结果显示: 当薄荷醇浓度在4%以内时对仿刺参具有良好的麻醉效果, 麻醉起效迅速, 复苏率为100%; 在麻醉体积分数为0.25%、0.5%、1%时, 对大规格幼参麻醉时间及复苏时间影响显著, 而对中小规格影响不显著; 水温11～19℃时, 随着温度的升高, 仿刺参幼参的麻醉时间从15.62 min±1.31 min 缩短到12.17 min±0.21 min, 但复苏时间从13.61 min±4.85 min 显著增加到28.10 min±7.35 min, 不同水温下薄荷醇对仿刺参幼参的麻醉效果差异显著(P<0.05), 当水温超过21℃时幼参麻醉状态出现异常, 不适宜进行麻醉。研究表明: 薄荷醇是一种对仿刺参安全有效的麻醉剂, 在水温11～21℃进行测量体长的实验中, 对体质量20 g以上的幼参适宜的麻醉体积分数为0.5%～1%, 对体质量20 g以下的幼参适宜的麻醉体积分数为0.25%～0.5%。 |
| 关键词: 仿刺参(Apostichopus japonicus) 薄荷醇 麻醉 温度 |
| DOI：10.11759/hykx20150826001 |
| 分类号: |
| 基金项目:山东省农业良种工程项目(2014-2016); 山东省科技发展计划项目(2014GNC111022); 国家海洋局海洋公益性行业科研专项经费项目(201305001-4); 山东省现代农业产业技术体系刺参产业创新团队建设项目(SDAIT-08-011-01); 山东省农业科技成果转化资金项目 |
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| Anesthetic effect of menthol on juvenile sea cucumber Apostichopus japonicus |
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HAN Sha,HU Wei,LI Cheng-lin,ZHAO Bin,YAN Fang
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| Abstract: |
| The aim of this study was to establish an anesthetic method for accurate body length measurement. We investigated the anesthetic effect of menthol on different-sized (large, 42.37 g±1.99 g; medium, 22.91 g±1.03 g; small, 12.09 g±1.51 g) Apostichopus japonicus juvenile sea cucumbers in different concentrations and at different water temperatures (11, 13, 15, 17, 19, 21℃). The results showed that the anesthetic effect of menthol took effect quickly and the recovery rate reached 100% at concentrations less than 4%. The anesthetic time and recovery time were significantly different for large individuals at concentrations of 0.25%, 0.5%, and 1%, but they were not significantly different for medium-sized and small ones. Within the temperature range from 11℃ to 19℃, the anesthetic time for sea cucumbers was shortened from 15.62 min±1.31 min to 12.17 min±0.21 min, while the recovery time increased from 13.61 min±4.85 min to 28.10 min±7.35 min. The anesthetic effect was significantly different at different temperatures (P<0.05). It was unfit for anesthetization because the status of the sea cucumber was abnormal when the temperature was higher than 21℃. These results suggest that, within the temperature range from 11℃ to 21℃, the effective anesthetic concentration of menthol is approximately 0.5% to 1% for individuals with body weight greater than 20 g and approximately 0.25% to 0.5% for individuals less than 20 g in body weight. |
| Key words: Apostichopus japonicus menthol anesthetization water temperature |
