| 引用本文: | 侯西坦,廖梅杰,李 彬,王印庚,张 正,陈贵平,荣小军,孙金生,范瑞用.刺参4个不同选育品系幼参对低盐胁迫的耐受及生理生化响应[J].海洋科学,2016,40(5):19-28. |
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| 刺参4个不同选育品系幼参对低盐胁迫的耐受及生理生化响应 |
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侯西坦1,2, 廖梅杰2, 李 彬2, 王印庚2, 张 正2, 陈贵平2, 荣小军2, 孙金生1, 范瑞用3
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1.天津师范大学 生命科学学院;2.中国水产科学研究院 黄海水产研究所, 青岛海洋科学与技术国家实验室 海洋渔业科学与食物产出过程功能实验室;3.青岛瑞滋海珍品发展有限公司
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| 摘要: |
| 由于黄河口区域盐度偏低且不稳定, 刺参(Apostichopus japonicus)的生长和存活受到严重威胁。为评估4个不同品系(日本刺参F2代RC-F2、抗病刺参F3代KC-F3、多刺刺参F2代DC-F2、青青刺参F1代QQ-F1)的低盐耐受力及其生理生化响应, 本实验以青岛野生群体(QW)为对照, 通过测定低盐胁迫下幼参的生长、存活以及非特异性免疫酶活力变化, 评价了4个刺参不同选育品系对低盐的耐受状况。结果表明, 在盐度17以下, 除了QQ-F1表现为正生长, 其他群体均为负生长。RC-F2、KC-F3、DC-F2、QQ-F1和QW的30天半致死盐度(LS50-30d)分别为15.02、15.19、16.48、14.26和15.13, QQ-F1显著低于其他4个群体; 盐度14时, 5个群体的半致死时间(ST50)分别为19.84 d、18.43 d、10.11 d、23.54 d和19.01 d, QQ-F1 的ST50显著长于其他4个群体。在低盐胁迫时QQ-F1的ACP、AKP、SOD、LZM酶指标的下降幅度显著低于其他4个群体。不同选育群体的低盐耐受力排序为QQ-F1>RC-F2>QW>KC-F3>DC-F2, QQ-F1群体表现出良好的低盐耐受能力, 在低盐度海域或盐度不稳定的海域具有良好的推广应用前景。本实验研究结果可为刺参健康养殖、良种性状评价、选育与推广等方面提供参考依据。 |
| 关键词: 刺参(Apostichopus japonicus) 低盐胁迫 特定生长率 半致死盐度 半致死时间 非特异性免疫酶 |
| DOI：10.11759/hykx20151030001 |
| 分类号: |
| 基金项目:国家十二五“863”项目 (2012AA10A412-4); 国家自然科学基金项目(31202016); 山东省农业良种工程重大项目“速生抗病耐高温刺参良种选育”; 国家948 项目(2014-Z13); 山东省自主创新成果转化专项(2013ZHZX2A0801) |
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| Response to low salinity of four strains of sea cucumber Apostichopus japonicus larvae |
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HOU Xi-tan,LIAO Mei-jie,LI Bin,WANG Yin-geng,ZHANG Zheng,CHEN Gui-ping,RONG Xiao-jun,SUN Jin-sheng,FAN Rui-yong
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| Abstract: |
| The salinity of the sea in estuarine areas is low and unstable, which threatens the survival and growth of sea cucumbers. To evaluate the low salinity tolerance of four sea cucumber (Apostichopus japonicus) strains (RC-F2, KC-F3, DC-F2, and QQ-F1), the growth, survival, and non-specific immune enzyme activities were monitored at seven salinity levels (13, 14, 15, 16, 17, 18, and 31); wild sea cucumber specimens from the Qingdao coast (QW) were used as controls. The RSGs (Specific Growth Rate) of all the strains, including the control, QW, decreased significantly under the stress of low salinity conditions. Only the QQ-F1 strain showed positive RSG at salinity 17. The LS50-30d of QQ-F1 was significantly lower than that of the other strains (RC-F2, KC-F3, DC-F2, QQ-F1, and QW were 15.02, 15.19, 16.48, 14.26, and 15.13, respectively). At salinity 14, the ST50 of QQ-F1 was significantly longer than that of the other strains (RC-F2, KC-F3, DC-F2, QQ-F1, and QW were 19.84d, 18.43d, 10.11d, 23.54d, and 19.01d, respectively). Although the enzyme activities of AKP, ACP, SOD, and LZM were decreased in all 5 species under low salinity conditions (salinity<18), the QQ-F1 strain maintained a comparatively higher enzyme activity level and showed higher overall tolerance to the stress of low salinity. Based on the growth, survival, and non-specific immune enzyme activities, the tolerance to low salinity of the 5 populations was ordered as follows: QQ-F1>RC-F2>QW>KC-F3>DC-F2. The high tolerance of QQ-F1 for low salinity suggest that it would be a good candidate for popularization of low salinity sea areas or unstable salinity coastal areas. These results could be helpful for healthy farming and breeding of sea cucumbers, as well as for evaluations of their character. |
| Key words: Apostichopus japonicus low salinity stress specific growth rate median lethal salinity median survival time non-specific immune enzyme activity |
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