引用本文:	黄勇,刘松,秦玉坤,邢荣娥,李克成,于华华,岳洋,李俨,李鹏程.壳聚糖碱性氨基酸衍生物的合成及抑菌活性研究[J].海洋科学,2017,41(1):24-29.

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壳聚糖碱性氨基酸衍生物的合成及抑菌活性研究
黄勇^1,2, 刘松¹, 秦玉坤¹, 邢荣娥¹, 李克成¹, 于华华¹, 岳洋^1,2, 李俨^1,2, 李鹏程¹
1.中国科学院海洋研究所;2.中国科学院大学

摘要:

利用接枝反应, 采用碱性氨基酸修饰壳聚糖, 制备壳聚糖赖氨酸衍生物、壳聚糖精氨酸衍生物、壳聚糖组氨酸衍生物。通过红外(FT-IR)、核磁(¹H-NMR)、元素分析(EA)对其进行表征, 并研究了不同壳聚糖氨基酸衍生物的抑菌活性。结果表明, 壳聚糖赖氨酸衍生物、壳聚糖精氨酸衍生物、壳聚糖组氨酸衍生物、壳聚糖对金黄色葡萄球菌的最低抑菌浓度(MIC)分别为320、160、320、640 μg/mL, 对大肠杆菌的最低抑菌浓度(MIC)分别为320、320、320、640 μg/mL, 三种壳聚糖氨基酸衍生物的抑菌活性均明显高于未修饰壳聚糖。通过引入碱性氨基酸增加壳聚糖的正电荷有利于提高其抑菌活性。

关键词: 壳聚糖碱性氨基酸衍生物抑菌活性

DOI：10.11759/hykx20160408001

分类号:

基金项目:中国科学院STS 计划项目(KFJ-SW-STS-143); 国家海洋公益性项目(201305016-2 、201405038-2); NSFC- 山东联合资助项目(U1406402-5)

Synthesis and antibacterial activities of basic amino acidmodified chitosan derivatives

HUANG Yong,LIU Song,QIN Yu-kun,XING Rong-e,LI Ke-cheng,YU Hua-hua,YUE Yang,LI Yan,LI Peng-cheng

Abstract:

In this study, three basic amino acids were used to modify chitosan to improve its antibacterial activity. Lysine-modified chitosan, arginine-modified chitosan, and histidine-modified chitosan were synthesized, and their structures were characterized using Fourier-transform infrared, magnetic resonance spectra (¹H-NMR), and elemental analysis. The results showed that the amino acids were successfully grafted onto chitosan. The antibacterial activities of these derivatives against Escherichia coli and Staphylococcus aureus were investigated. The MIC of lysine-modified chitosan, arginine-modified chitosan, and histidine-modified chitosan on S. aureus was 320, 160, 320, and 640 μg/mL, respectively, and the MIC on E.coli was 320, 320, 320, and 640 μg/mL, respectively. All three basic amino acid-modified chitosan derivatives exhibited better antibacterial activity than chitosan.

Key words: Chitosan basic amino acids derivatives antibacterial activity