| 摘要: |
| 半滑舌鳎(Cynoglossus semilaevis)是中国重要的海水养殖鱼类品种, 目前对于其肾脏结构与细胞种类的研究较少。本研究以半滑舌鳎肾脏为研究对象, 通过切片技术、免疫组织化学与显微镜观察, 描述半滑舌鳎肾脏结构与细胞分布情况。结果显示, 半滑舌鳎的肾脏可以分为头肾与体肾两部分; 头肾较小, 具有独立的结构, 位于肾脏前部, 靠近脑, 形态上呈分叉状; 头肾由淋巴髓样细胞构成, 无肾小球等分泌结构, 免疫细胞丰富, 包括淋巴细胞、单核细胞、颗粒细胞, 是半滑舌鳎免疫细胞的主要生发部位之一, 是免疫系统的重要组成; 半滑舌鳎的体肾与头肾后部分叉处相连, 呈纺锤状, 紧贴腹腔背部, 向后延伸; 体肾主要由肾单位组成, 有丰富的肾小囊、近曲小管、远曲小管和集合管结构, 表明其具有排泄功能。 |
| 关键词: 半滑舌鳎(Cynoglossus semilaevis) 肾脏 组织结构 石蜡切片 超薄切片 |
| DOI：10.11759/hykx 20160622001 |
| 分类号: |
| 基金项目:国家自然科学基金项目(31672692); 山东省自主创新及成果转化专项(2014ZZCX06204); 鳌山科技创新计划(2015ASKJ02) |
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| Histological structure and cell distribution of the tongue sole (Cynoglossus semilaevis) kidney |
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SUN Bin,SUN Jin-sheng,ZHANG Li-na,XIAO Peng
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| Abstract: |
| Half-smooth tongue sole (Cynoglossus semilaevis) is an important aquaculture species in China. Here, we illustrated the structure of the tongue sole kidney by observing paraffin sections and ultrathin sectioned samples by light and transmission electron microscopy. The tongue sole kidney was anatomically divided into the head and trunk kidneys. The head kidney was a biforked structure in front of the trunk kidney. The head kidney was located on top of the heart, close to the rear of the head. Some blood cells and many immune cells were found in the head kidney, such as lymphocytes, monocytes, and granulocytes. We inferred that the tongue sole head kidney played an important role in the immune response and hemopoiesis. The trunk kidney was located immediately behind where the two head kidney branches joined. The trunk kidney was stretched along the spine in the abdominal cavity and was composed of many glomerular secretory structures and renal units, indicating that it possesses an excretory function. |
| Key words: Cynoglossus semilaevis kidney histological structure paraffin section ultrathin section |
