| 摘要: |
| 采用超声波法制备微藻DHA油纳米乳, 研究了微藻DHA油纳米乳真空冷冻干燥工艺, 并对其粉末进行了表征。研究结果表明, 当冻干曲线为: 预冻: –40℃维持2 h, 真空度为0.2 mbar; 升华干燥: –30℃维持12 h, 真空度为0.2 mbar, –20℃维持2.5 h, 真空度为0.15 mbar; 解析干燥: 25℃维持3 h, 真空度为0.15 mbar,且油相与冻干保护剂的质量比为1︰4, 冻干保护剂选择葡萄糖︰ 甘露醇质量比为1︰3 时, 制备的纳米乳粉末颗粒外观呈乳白色、均匀细腻, 复溶速度在10s内, 冻干前粒径为(175.6±22.1)nm, 多分散系数(PDI)为(0.15±0.62), 冻干后粒径为(211.1±23.5)nm, PDI 为(0.152±0.116), 差异不大, 依旧在纳米范围内, 不仅保证了DHA的长期稳定性, 还扩大了DHA 的应用范围, 有望成为DHA的新型纳米载药系统。 |
| 关键词: 微藻DHA 油 纳米乳 真空冷冻干燥 |
| DOI：10.11759/hykx20160311001 |
| 分类号: |
| 基金项目:厦门南方海洋研究中心项目 (No. 14CZP028HJ02); 国家自然科学基金项目(No. 41476148); 海洋经济创新发展区域示范项目(12PYY001SF08) |
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| Study on the vacuum freeze-drying process on microalgae oil nanoemulsion powder |
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LIU Wei-wei,WU Peng,WANG Zhao-kai,LIN Xiang-zhi
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| Abstract: |
| This study explores the vacuum freeze-drying process and characterizes microalgae DHA oil nanoemulsion nanopowder, which is synthesized via ultrasonic. Result shows that the nanopowder is milky white and delicate with a constant dissolution rate of 10 s under the following conditions of the freeze-drying curve with oil phase to freeze-drying protective additive ratio of 1︰4 and glucose to mannitol ratio of 1︰3: (1) Pre-freezing at ?40°C for 2 h under 0.2 mbar pressure; (2) Sublimation drying at –30℃ for 12 h under 0.2 mbar pressure followed by drying at –20℃ under 0.15 mbar pressure for 2.5 h; (3) Analytical drying at 25℃ for 3 h under 0.15 mbar pressure. Before freeze-drying, the particle size and polydispersity coefficient were (175.6±22.1) and (0.15±0.62) nm, respectively, and these values changed to (211.1±23.5) and (0.152±0.116), respectively, after freeze-drying, indicating that the difference between these values is small and in the range of nanometers. The vacuum freeze-drying technology not only ensures the long-term stability of DHA but also expands the scope of its application. DHA is therefore expected to become a new nano-loaded drug system. |
| Key words: microalgae DHA oil nanoemulsion vacuum freeze-drying |
