| 摘要: |
| 滨海湿地因受河流和海水的交互作用, 其土壤氧化还原电位及营养元素等条件复杂且处于不断变化之中, 为了阐明此种环境下不同水分条件对滨海芦苇湿地土壤微生物多样性的影响, 尤其是对铁还原菌群落结构的影响, 作者以黄河三角洲滨海湿地为对象, 研究了淹水和非淹水芦苇湿地表层(0～30 cm)土壤理化特性、微生物多样性(采用T-RFLP技术)和培养条件下湿地土壤微生物的Fe(III)还原能力的变化。结果表明, 淹水芦苇湿地土壤有机碳、全氮、盐度、Fe(II)含量和pH值显著(P<0.05)高于非淹水芦苇湿地土壤, 而淹水芦苇湿地土壤铵态氮含量显著(P<0.05)低于非淹水芦苇湿地。淹水芦苇湿地土壤主要优势细菌和古菌分别为铬还原菌属(Alishewanella)、纤维单胞菌属(Cellulomonas)、鞘脂单胞菌属(Sphingomonas) 、假单胞菌属(Pseudomonas)和甲烷杆菌属(Methanobacterium) 、甲烷八叠球菌属(Methanosarcina); 而纤维单胞菌属、食酸菌属(Acidovorax) 、地杆菌属(Geobacter) 、硫杆菌属(Thiobacillus)和甲烷八叠球菌属分别为非淹水芦苇湿地土壤主要优势细菌和古菌。淹水芦苇湿地土壤主要优势铁还原菌为副球菌属(Paracoccus)、地杆菌属、铬还原菌属和硫单胞菌属(Thiomonas), 而非淹水芦苇湿地土壤为地杆菌属和固氮螺菌属(Azospira)。说明淹水能增加芦苇湿地土壤细菌、古菌和铁还原菌的多样性。培养条件下, 淹水芦苇湿地土壤铁还原菌的还原Fe(III)能力在培养后期(25d后)低于非淹水芦苇湿地, 说明淹水条件在一定程度上抑制了芦苇湿地土壤铁还原菌的铁还原能力。 |
| 关键词: 水分条件 芦苇湿地 微生物群落 铁还原菌 多样性 |
| DOI：10.11759//hykx20161018001 |
| 分类号: |
| 基金项目:国家自然科学基金面上项目(41371257、41573071); 国家自然科学基金青年项目(41401285、31600370); 中国科学院重点部署项目(KZZD-EW-14); 中国科学院前沿科学重点研究项目(QYZDJ-SSWDQC015);泰山学者青年专家项目(tsqn20161054) |
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| Effects of water conditions on the diversity of soil microbial communities in the coastal reed wetlands |
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ZHANG Hong-xia,ZHENG Shi-ling,WEI Wen-chao,WANG Bing-chen,WANG Ou-mei,LIU Fang-hua
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| Abstract: |
| River-sea water interactions in coastal wetlands dramatically affect soil redox potential and cause variations in nutrient elements. In this study, we analyzed the changes in physicochemical properties of soil, diversity in the microbial community, and the Fe(III) reduction activity of microorganisms in reed wetlands of the Yellow River Delta under different water conditions. The results showed that the salinity, pH value, and concentrations of organic carbon, total nitrogen, and Fe(II) in the soil were higher in the flooded reed wetlands as compared with the ammonium concentration. The genera Alishewanella, Cellulomonas, Sphingomonas and Pseudomonas were the dominated bacteria in flooded reed wetlands, whereas Methanosarcina and Methanobacterium were the dominant archaea. Further, the dominated bacteria and archaea in the non-flooded reed wetlands were Cellulomonas, Acidovorax, Geobacter, Thiobacillus, and Methanosarcina, respectively. A shift in the iron-reducing bacterial community structure was also observed in the enrichment samples from flooded and non-flooded reed wetlands. Paracoccus, Geobacter, Alishewanella, and Thiomonas were the dominant iron-reducing bacteria in the flooded reed wetlands, and the dominant genera of iron-reducing bacteria in the non-flooded reed wetlands were Geobacter and Azospira. The diversity of soil bacteria, archaea, and iron-reducing bacteria was higher in the flooded reed wetlands. The Fe(III) reduction activity of iron-reducing bacteria in the reed wetlands was lessened to a certain extent by perennial flooded conditions. |
| Key words: water condition reed wetlands microbial community iron-reducing bacteria diversity |
